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Question (60899) | Anti-AKT1 (phospho S473) antibody [EP2109Y] (ab81283)

I am a Ph.D student studying at the University of Malta and I have some queries regarding my recent order:Anti-S6K antibody [E175] (ab32359) and Anti-AKT1 (phospho S473) antibody [EP2109Y] (ab81283).

To which S6K isoform (S6K1 and/or S6K2) does Anti-S6K antibody [E175] (ab32359) react to?

Moreover should the Anti-AKT1 (phospho S473) antibody [EP2109Y] (ab81283) stain normal tissue as I have been having the same intensities in tumour and in normal tissue. I have tried the Akt1 on cervix, breast and prostate tumours with surrounding normal tissue and in all cases it was difficult to determine between normal and tumour by the intensity.

I would like to thank you while awaiting for your reply

Thank you for contacting us.

The exact epitope is proprietary, but the immunogen sequence is approximately 20 amino acids long and maps within amino acids 400-440 of p70 S6 Kinase of human origin.Thismeans, that both isoforms will be detected as they differ on the N-terminus.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.