Western blot abreview for Anti-AKT1 (phospho S473) antibody [EP2109Y]

Average
Abreviews
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (macrophage)
Specification macrophage
Treatment 0.1 ug/ml LPS 15 min
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time 14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution 1/1000

Secondary antibody

Name Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) (DyLight 680), Polyclon
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Other

Detection

Detection method Odyssey
Bands Specific: 60 kDa
Exposure 5 minute(s) and 0 second(s)
Username

Abcam user community

Verified customer

Submitted Dec 12 2014