Western blot abreview for Anti-AKT1 (phospho S473) antibody [EP2109Y]

Average
Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (macrophage)
Specification
macrophage
Treatment
0.1 ug/ml LPS 15 min
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) (DyLight 680), Polyclon
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Other

Detection

Detection method
Odyssey
Bands
Specific: 60 kDa
Exposure
5 minute(s) and 0 second(s)
Username

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Submitted Dec 12 2014

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