Immunocytochemistry/ Immunofluorescence abreview for Anti-AKT1 (phospho S473) antibody [EP2109Y]

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Peritonei tumor)
Permeabilization Yes - 0.3%Triton X 100
Specification Peritonei tumor
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde

Other product details

Incubation time 2 hour(s) and 0 minute(s)
Dilution 1/500

Secondary antibody

Dilution 1/1000
Name Non-Abcam antibody was used: Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Flu
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647

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Submitted Jan 19 2016