Western blot abreview for Anti-AKT1 (phospho S473) antibody [EP2109Y]

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Abreviews
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Application Western blot
Sample Human Cell lysate - whole cell (Epithelial OVCA cell line)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 100 µg
Treatment 0- 200µM Perifosine 24 hr
Specification Epithelial OVCA cell line
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time 4 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% milk
Dilution 1/1000

Secondary antibody

Dilution 1/10000
Name Non-Abcam antibody was used: Anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method ECL Western Blotting Substrate
Exposure 5 minute(s) and 0 second(s)
Bands Specific: 65 kDa
Positive control Untreated OVACAR cell extract
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Submitted Jul 13 2016