Western blot abreview for Anti-AKT1 (phospho S473) antibody [EP2109Y]

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Abreviews
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Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293 cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
HEK 293 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/4000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 56 kDa
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Submitted Sep 23 2016

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