AKT1 (phospho S473) antibody (ab28821)

Product Name 

AKT1 (phospho S473) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to AKT1 (phospho S473)

Immunogen 

Synthetic phospho-peptide derived from human AKT1 around the phosphorylation site of Serine 473.

Reacts with 
(species key)

Hu, Ms

Specificity 

ab28821 is specific for phosphorylated AKT1(S473). It may also recognise phospho-AKT2 (S473)and phospho-AKT3 (S473) due to sequence homology.

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: 1/2000.
IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol (see recommended protocol).
WB: Use at an assay dependent dilution from (PubMed:16918991). BSA as a blocking agent is recommended for this antibody.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Breast carcinoma and human heart tissue lysate.

Cellular localization 

Cytoplasmic, and nuclear after activation by integrin linked protein kinase 1 (ILK1).

Research areas 

Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> AKT
Chromatin and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
Neuroscience >> Neurology process >> Neural Signal Transduction

Relevance 

The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene (referenced from entrez gene).

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Purification notes 

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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References for AKT1 (phospho S473) antibody (ab28821)

  This product has been used in:

Takehara A et al. Novel tumor marker REG4 detected in serum of patients with resectable pancreatic cancer and feasibility for antibody therapy targeting REG4. Cancer Sci 97:1191-7 (2006). PubMed: 16918991

If you publish research using ab28821 please let us know so that we can cite the reference on this datasheet.

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