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Staining of phospho-AKT1 on sections of human breast carcinoma, using ab47261 diluted 1/50. In the right panel the antibody was pretreated with the corresponding blocking phosphopeptide.
All lanes : Anti-AKT1 (phospho S473) antibody (ab47261) at 1/100 dilution
Lane 1 : Heat shock treated HeLa cells extract
Lane 2 : HeLa cells extract
Predicted band size : 56 kDa
Observed band size : 56 kDa
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