AKT1 (phospho S473) antibody (ab47261)

Product Name 

AKT1 (phospho S473) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to AKT1 (phospho S473)

Immunogen 

Synthetic phosphopeptide designed around serine 473 of Human AKT1 (QFS^PYS)
BLAST 'QFSYS' with or

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat
(Due to sequence homology)

Specificity 

ab47261 is specific for phosphorylated AKT1(S473). It may also recognise phospho-AKT2 (S473)and phospho-AKT3 (S473) due to sequence homology.

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/2000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Breast carcinoma.
HeLa cells.

Cellular localization 

Cytoplasmic, and nuclear after activation by integrin linked protein kinase 1 (ILK1).

Research areas 

Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> AKT
Chromatin and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
Neuroscience >> Neurology process >> Neural Signal Transduction

Relevance 

The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene (referenced from entrez gene).

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

See below for AKT1 (phospho S473) antibody images, references, products related to ab47261 and other tools.

AKT1 (phospho S473) antibody images:

Customer reviews (feedback) regarding AKT1 (phospho S473) antibody

Customer FAQs regarding AKT1 (phospho S473) antibody

Protocols for AKT1 (phospho S473) antibody

Price and availability of products related to AKT1 (phospho S473) antibody

AKT1 (phospho S473) antibody - related products: