Anti-AKT1 (phospho S473) antibody (ab8932)

- AKT1 (phospho S473) antibody (ab8932)

Normal breast tissue. Akt is weakly phosphorylated in normal tissue in the breast. The phosphorylated Akt is clearly localised in the cytoplasm.

The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.

- AKT (phospho S473) antibody (ab8932)

Akt is phosphorylated on Ser 473 in breast tumor. The staining is much stronger than the weak basal level of phosphorylation in normal breast. The staining is cytoplasmic, as in normal tissue.

The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.

This image is a magnification of the accompanying breast tumor image.

Western blot - Anti-AKT1 (phospho S473) antibody (ab8932)

Predicted band size : 56 kDa


ab8932 at a 1:200 dilution in Western Blot staining nuclear extract from cells infected with adenovirus expressing nuclear-targeted Akt kinase.

Immunocytochemistry/ Immunofluorescence - AKT1 (phospho S473) antibody (ab8932)

Confocal image of ab8932 at a 1:40 dilution staining cardiomyocytes infected with wild-type Akt in conjunction with a texas-red conjugated phalloidin to label filamentous actin in the cardiomyocytes.

Immunocytochemistry/ Immunofluorescence - AKT1 (phospho S473) antibody (ab8932)

Confocal image of ab8932 at a 1:40 dilution staining cardiomyocytes infected with wild-type Akt in conjunction with a texas-red conjugated phalloidin to label filamentous actin in the cardiomyocytes.

- AKT1 (phospho S473) antibody (ab8932)

Akt is phosphorylated on Ser 473 in breast tumor. The staining is much stronger than the weak basal level of phosphorylation in normal breast. The staining is cytoplasmic, as in normal tissue.

The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.