Anti-AKT1 (phospho S473) antibody (ab8932)

I am intrerested in getting the anti AKT antibodies: ab 8932 and other AKT antibodies for Immunocitochemistry to look at endogenous AKT (rather than overexpressing cells ie transfected/ infected cells)-which antibodies would you recommend? and could you please send me references where I can see the confocal images using these antibodies?

ANSWER:

Thank you for your email. Are you specifically looking for a phospho S473 AKT antibody? If yes, we currently have two: ab8932 and ab4802. For both all the information that we have is located on the online datasheets. When we know of references that feature the use of an antibody, we cite them on the online datasheets. If you have any further questions, please contact us again.

Lot - 58039 Dear Sir/Madam, I recently purchased the above antibody which worked well when using control tissue (breast cancer). I have since run 2 batches of various tumour tissues with some weakly positive results. Surprisingly though the positive controls (breast cancer, 1/100 concentration of antibody) have been completely negative on both these occasions suggesting that the antibody is no longer working. I had aliquotted and frozen the antibody at -20 as recommended although at delivery the box contained only 1 ice pack which wasn't frozen. I presume the Ab is very temperature dependant and has a very short shelf-life if it isn't frozen. Meanwhile I have lost 3 days of work and some extremely important human tissue. Could you please send me a replacement ensuring it is adequately packaged and advise me as to the best way of handling/storing this antibody to avoid loss of function.

The antibody is staining the vascular endothelium, is this expected.

Yours truly,

ANSWER:

Thank you for your enquiry. We are sorry to hear that you have problem with this antibody.

We have searched our database and found that this is a popular selling product and your feedback is the first we have received about it not working.

The antibody should be aliquotted immediately after arrival and store at -20°C or -80°C.Avoid repeated freeze / thaw cycles and use one fresh aliquot for each experiment.

We can offer you a replacement vial free of charge. For your information, your new order number is 48098. Please do let us know how you are getting on with this vial.