Anti-AKT1 (phospho T308) antibody (ab4796)

Overview

• Product nameAnti-AKT1 (phospho T308) antibodySee all AKT1 primary antibodies ...
• Description
  Rabbit polyclonal to AKT1 (phospho T308)
• SpecificityThe sequence around T308 is highly similar to the homologous region in AKT2 and AKT3, thus this antibody may cross react with these proteins if phosphorylated at the corresponding threonine residue.
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide derived from a region of AKT that contains threonine 308.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 50% Glycerol, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified from rabbit serum by epitope-specific chromatography. Any reactivity towards the non-threonine phosphorylated AKT protein has been eliminated through a series of preabsorption steps.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Cancer
  • Signal transduction
  • Protein phosphorylation
  • Serine/threonine kinases
  • AKT

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • PKB / AKT

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Apoptosis
  • Nuclear

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

Applications

Target

• FunctionPlays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
• Tissue specificityExpressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
• Involvement in diseaseDefects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
  Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].
  Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).
• Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
  Contains 1 AGC-kinase C-terminal domain.
  Contains 1 PH domain.
  Contains 1 protein kinase domain.
• DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. The PH domain mediates interaction with TNK2 and Tyr-176 is also essential for this interaction.
  The AGC-kinase C-terminal mediates interaction with THEM4.
• Post-translational
  modificationsPhosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.
  Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
• Cellular localizationCytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.

Target information above from: UniProt accession P31749 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 207 Human
  • Entrez Gene: 11651 Mouse
  • Entrez Gene: 24185 Rat
  • Omim: 164730 Human
  • SwissProt: P31749 Human
  • SwissProt: P31750 Mouse
  • SwissProt: P47196 Rat
  • Unigene: 525622 Human

  • Unigene: 6645 Mouse
  • Unigene: 11422 Rat

  see all

• Alternative names
  AKT 1 antibodyAKT antibodyAKT1 antibody

  AKT1_HUMAN antibodyC AKT antibodyC AKT antibodyCAKT antibodyMGC9965 antibodyMGC99656 antibodyMurine thymoma viral (vakt) oncogene homolog 1 antibodyOncogene AKT1 antibodyPKB Akt antibodyPKB alpha antibodyPKB antibodyPKB antibodyPKB-ALPHA antibodyPKBalpha antibodyPRKBA antibodyProtein Kinase B Alpha antibodyProtein kinase B antibodyProto-oncogene c-Akt antibodyRAC Alpha antibodyRAC alpha serine/threonine protein kinase antibodyRAC antibodyRAC PK Alpha antibodyRac protein kinase alpha antibodyRAC Serine/Threonine Protein Kinase antibodyRAC-alpha serine/threonine-protein kinase antibodyRAC-PK-alpha antibodyV AKT Murine Thymoma Viral Oncogene Homolog 1 antibodyvAKT Murine Thymoma Viral Oncogene Homolog 1 antibodyVAKT Murine Thymona Viral Oncogene 1 antibody

  see all

Anti-AKT1 (phospho T308) antibody images

• 

  Western blot - AKT1 (phospho T308) antibody (ab4796)
  
  
  Predicted band size : 56 kDa
  
  
  Extracts of NIH-3T3 cells, serum starved overnight and treated for 15 minutes with the PDGF concentrations indicated, were resolved by SDS-PAGE on a 4-20% Tris-glycine gel. The proteins were then transferred to PVDF membrane. Membranes were incubated with 0.5 µg/mL of ab4796. After washing, membranes were incubated with goat F(ab) 2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. Data show clear activation of PKB/Akt phosphorylation at threonine 308 in these cells with PDGF treatment.
• 

  Immunohistochemistry (PFA perfusion fixed frozen sections) - AKT1 (phospho T308) antibody (ab4796)Image courtesy of Dr S Pezet, CARD institute, KCL, London, UK
  AKT Thr 308 antibody (ab4796) at a dilution of 1/100 (2 X o/n incubation at RT; ABC amplification). Cytoplasmic and nuclear staining was observed in rat spinal cord in [A] motorneurons (ventral horn); 40x objective and [B] Lateral cuneate nucleus 40x objective. Staining performed in free floating IHC on 30µm saggital spinal cord sections. Find detailed information regarding the protocol in the Specific Protocol link.

Protocols

• Peptide Competition Experiment
• Protocol for perfusion fixed tissue IHC (DAB)
• Western blotting protocol