Overview

  • Product nameAnti-AKT1 (phospho T308) antibodySee all AKT1 primary antibodies ...
  • Description
    Rabbit polyclonal to AKT1 (phospho T308)
  • SpecificityThe sequence around T308 is highly similar to the homologous region in AKT2 and AKT3, thus this antibody may cross react with these proteins if phosphorylated at the corresponding threonine residue.
  • Tested applicationsIHC-FrFl, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from a region of AKT that contains threonine 308.

Properties

Applications

Our Abpromise guarantee covers the use of ab4796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl
WB
  • Application notesWB: Use at a concentration of 0.5 - 1.0 µg/ml. Detects a band of approximately ~60 kDa (predicted molecular weight: 56 kDa).
    Can be blocked with AKT peptide - phospho T308 (phospho and non-phospho pair).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
    • Tissue specificityExpressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
    • Involvement in diseaseDefects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
      Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].
      Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
    • DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. The PH domain mediates interaction with TNK2 and Tyr-176 is also essential for this interaction.
      The AGC-kinase C-terminal mediates interaction with THEM4.
    • Post-translational
      modifications
      Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.
      Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
    • Cellular localizationCytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • AKT 1 antibody
      • AKT antibody
      • AKT1 antibody
      • AKT1_HUMAN antibody
      • C AKT antibody
      • CAKT antibody
      • MGC9965 antibody
      • MGC99656 antibody
      • Murine thymoma viral (vakt) oncogene homolog 1 antibody
      • Oncogene AKT1 antibody
      • PKB Akt antibody
      • PKB alpha antibody
      • PKB antibody
      • PKB antibody
      • PKB-ALPHA antibody
      • PKBalpha antibody
      • PRKBA antibody
      • Protein Kinase B Alpha antibody
      • Protein kinase B antibody
      • Proto-oncogene c-Akt antibody
      • RAC Alpha antibody
      • RAC alpha serine/threonine protein kinase antibody
      • RAC antibody
      • RAC PK Alpha antibody
      • RAC Serine/Threonine Protein Kinase antibody
      • RAC-alpha serine/threonine-protein kinase antibody
      • RAC-PK-alpha antibody
      • V AKT Murine Thymoma Viral Oncogene Homolog 1 antibody
      • vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody
      • VAKT Murine Thymona Viral Oncogene 1 antibody
      see all

    Anti-AKT1 (phospho T308) antibody images



    • Predicted band size : 56 kDa


      Extracts of NIH-3T3 cells, serum starved overnight and treated for 15 minutes with the PDGF concentrations indicated, were resolved by SDS-PAGE on a 4-20% Tris-glycine gel. The proteins were then transferred to PVDF membrane. Membranes were incubated with 0.5 µg/mL of ab4796. After washing, membranes were incubated with goat F(ab) 2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. Data show clear activation of PKB/Akt phosphorylation at threonine 308 in these cells with PDGF treatment.
    • AKT Thr 308 antibody (ab4796) at a dilution of 1/100 (2 X o/n incubation at RT; ABC amplification). Cytoplasmic and nuclear staining was observed in rat spinal cord in [A] motorneurons (ventral horn); 40x objective and [B] Lateral cuneate nucleus 40x objective. Staining performed in free floating IHC on 30µm saggital spinal cord sections. Find detailed information regarding the protocol in the Specific Protocol link.

    References for Anti-AKT1 (phospho T308) antibody (ab4796)

    This product has been referenced in:
    • Sei Y  et al. Epistatic and functional interactions of catechol-o-methyltransferase (COMT) and AKT1 on neuregulin1-ErbB signaling in cell models. PLoS One 5:e10789 (2010). WB ; Human . Read more (PubMed: 20520724) »

    See 1 Publication for this product

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    It recognises AKT1 and has not been tested for its cross reactivity with AKT 2 and 3.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"