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Synthetic peptide corresponding to Mouse AKT1 aa 302-314.
(Peptide available as
Our Abpromise guarantee covers the use of ab5626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. PubMed: 21785161|
|WB||Use a concentration of 10 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).Can be blocked with AKT1 peptide (ab5851).|
Immunohistochemical analysis of rat tongue tissue, staining AKT1 (phospho T308) with ab5626.
Tissue was fixed with Bouin's fixative and blocked with 0.2% Casein in sodium carbonate buffer (pH 9.6) for 30 minutes at 22°C; antigen retrieval was by heat mediation in a Tris-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/150 in 10% goat serum + 1% BSA + 0.05 thiomerosal) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.
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