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Synthetic peptide within Human AKT1 (phospho T308). The exact sequence is proprietary.
Our Abpromise guarantee covers the use of ab66134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 - 2 µg/ml.|
|ELISA||Use a concentration of 0.01 - 0.1 µg/ml.|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 60 kDa.|
|IP||Use a concentration of 2 - 5 µg/ml.|
1 µg of a peptide was blotted onto a nitrocellulose membrane followed by ab66134 at 1/2000 dilution. Upper Panel: Dot 1: AKT1(pT308) peptide, Dots 2,3,4: unrelated phosphopeptides. Lower Panel: Dot 1: non phosphopeptide AKT1, Dots 2,3,4: unrelated non-phosphopeptides.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"