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Phospho-Akt (Thr-34) synthetic peptide (coupled to carrier protein) corresponding to amino acid residues around threonine 34 of human Akt.
Our Abpromise guarantee covers the use of ab23509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 56 kDa.|
Immunohistochemical analysis of frozen mouse embryo tissue, staining TNF alpha (green) with ab23509.
Tissue was fixed with paraformaldehyde, permeabilized with saponin and blocked with 10% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/50 in diluent) for 24 hours at 4C. An AlexaFluor®488-conjugated donkey anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody.
Red staining = Troponin; Blue staining = DAPI
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