Overview

  • Product name
    AKT2 ELISA Kit
    See all AKT2 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    NIH 3T3 8 3.6%
    Inter-assay
    Sample n Mean SD CV%
    NIH 3T3 3 7.3%
  • Sample type
    Cell culture supernatant, Cell culture extracts, Tissue Extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    5 pg/ml
  • Range
    78.13 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture media 105 103% - 107%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Abcam’s AKT2 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of AKT2 protein in cell culture supernatant and cell and tissue extract samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


     


    Sensitivity:


    Samples diluted in Sample Diluent NS: 7.6 pg/mL


    Samples diluted in 1X Cell Extraction Buffer PTR: 5 pg/mL


     

  • Notes

    AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinases. These kinases regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Specifically, AKT2 seems to be the principal isoform responsible for the regulation of glucose uptake. The AKT kinases mediate these processes through serine and/or threonine phosphorylation of a range of downstream substrates.

    Human and rat AKT2 exhibit 98.1% and 98.5% amino acid identity to mouse AKT2, respectively.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab208986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of AKT2 were measured in duplicates, interpolated from the AKT2 standard curve and corrected for sample dilution. Undiluted samples are as follows: cell culture 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AKT2 concentration was determined to be 1.3 ng/mL in RPMI base media.

  • The concentrations of AKT2 were measured in duplicate and interpolated from the AKT2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AKT2 concentration was determined to be 0.74 ng/mL in NIH 3T3 extract, 1.45 ng/mL in C2C12 extract, 4.47 ng/mL in MCF7 extract, and 1.85 ng/mL in PC12 extract.

Protocols

References

ab208986 has not yet been referenced specifically in any publications.

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