![Western blot - AKT2 antibody [95C657] (ab13655) image](#ab13655.jpg)
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Product name
Anti-AKT2 antibody [95C657]
See all AKT2 products (19) ...
Description
Mouse monoclonal [95C657] to AKT2
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: VANSLKQRAPGEDPMDYK, corresponding to amino acids 106-123 of Human AKT2
VANSLKQRAPGEDPMDYK
Positive control
Human placenta, HL60 whole cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
95C657
Isotype
IgM
Research areas
Metabolism >> Types of disease >> Heart disease
Metabolism >> Types of disease >> Cancer
Metabolism >> Types of disease >> Diabetes
Cardiovascular >> Heart >> Cardiac metabolism
Cardiovascular >> Atherosclerosis >> Diabetes associated
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> AKT
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
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Western blot - AKT2 antibody [95C657] (ab13655)
![Western blot - AKT2 antibody [95C657] (ab13655) image](/ps/datasheet/Images/13/ab13655/ab13655.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab13655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 56 kDa).
Target
Function
General protein kinase capable of phosphorylating several known proteins.
Tissue specificity
Expressed in all human cell types so far analyzed.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylation on Thr-309 and Ser-474 is required for full activity.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
Target information above from: UniProt accessionP31751
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AKT 2 antibody
- Akt2 antibody
- AKT2_HUMAN antibody
see all
Database links
- Entrez Gene: 208 Human
- Omim: 164731 Human
- SwissProt: P31751 Human
- Unigene: 631535 Human
Anti-AKT2 antibody [95C657] images:
Western blot - AKT2 antibody [95C657] (ab13655)
Predicted band size : 56 kDa
Western blot analysis of Akt2 using ab13655 at 1/1000 against 30 µg of human placenta lysate.
References for Anti-AKT2 antibody [95C657] (ab13655)
This product has been referenced in:
- Glienke W et al. The dual PI3K/mTOR inhibitor NVP-BGT226 induces cell cycle arrest and regulates Survivin gene expression in human pancreatic cancer cell lines. Tumour Biol : (2011). WB; Human.Read more (PubMed: 22170433) »
See 1 publication for this product
Publishing research using ab13655? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"