Overview

  • Product nameAnti-AKT2 antibody
    See all AKT2 primary antibodies
  • Description
    Rabbit polyclonal to AKT2
  • Tested applicationsELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human)(C terminal).

  • Positive control
    • A431 or 3T3 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab5920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.1 - 1 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).
IP Use 3-5µg for 106 cells.

Target

  • FunctionGeneral protein kinase capable of phosphorylating several known proteins.
  • Tissue specificityExpressed in all human cell types so far analyzed.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation on Thr-309 and Ser-474 is required for full activity.
    Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Information by UniProt
  • Database links
  • Alternative names
    • AKT 2 antibody
    • Akt2 antibody
    • AKT2_HUMAN antibody
    • HIHGHH antibody
    • murine thymoma viral (v-akt) homolog-2 antibody
    • PKB antibody
    • PKB antibody
    • PKB beta antibody
    • PKB beta antibody
    • PKBB antibody
    • PKBBETA antibody
    • PRKBB antibody
    • PRKBB antibody
    • Protein kinase Akt 2 antibody
    • Protein kinase Akt-2 antibody
    • Protein kinase B beta antibody
    • RAC BETA antibody
    • RAC beta serine threonine protein kinase antibody
    • RAC PK beta antibody
    • Rac protein kinase beta antibody
    • Rac protein kinase beta antibody
    • RAC-BETA antibody
    • RAC-beta serine/threonine-protein kinase antibody
    • RAC-PK-beta antibody
    • V AKT Murine Thymoma Viral Oncogene Homolog 2 antibody
    see all

Anti-AKT2 antibody images



  • Predicted band size : 56 kDa


    Western blot analysis of anti-AKT2 on the whole cell lysates derived from brain, heart, muscle, liver, spleen, thymus, testes, kidney, and lung, respectively.

References for Anti-AKT2 antibody (ab5920)

This product has been referenced in:
  • Nijholt IM  et al. Neuronal AKAP150 coordinates PKA and Epac-mediated PKB/Akt phosphorylation. Cell Signal 20:1715-24 (2008). WB ; Mouse . Read more (PubMed: 18565730) »

See 1 Publication for this product

Product Wall

Thank you for contacting us.

All the products you shortlisted seem to be suitable for your work, however, looking at the availability of those products, I would recommend the following one that are currently in stock:
Anti-AKT1 (N-term)ant...

Read More

The antibodies of AKT 1, 2, 3 are very specific. The phospho-specific antibody is specific as well. No cross reactivity to each other.

Thank you so much for your patience, I have only just heard back from the antibody's originator. Ab5920 only recognizes AKT2. If you have any more question, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"