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AKT2 protein (ab42593)

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Overview

Product name

AKT2 protein
See all AKT2 products (6) ...

Protein description

Recombinant full length protein (Human) with an N terminal His tag. M.Wt 57 kDa. Expression system: Baculovirus infected Sf9 cell expression system.

Expression host

Baculovirus infected sf9 cells

Properties

Purity

> 90% by SDS-PAGE

Biological activity

Akt2 is inactive and can be activated by upstream kinase PDK1.

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

Concentration

Concentration information loading...

Research areas

Metabolism >> Types of disease >> Heart disease
Metabolism >> Types of disease >> Cancer
Metabolism >> Types of disease >> Diabetes
Cardiovascular >> Heart >> Cardiac metabolism
Cardiovascular >> Atherosclerosis >> Diabetes associated
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> AKT
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT

Applications

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Our Abpromise guarantee covers the use of ab42593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Inhibition Assay

     

Application notes

Inhib: Use at an assay dependent dilution. Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Protein info

Function

General protein kinase capable of phosphorylating several known proteins.

Tissue specificity

Expressed in all human cell types so far analyzed.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.

Post-translational
modifications

Phosphorylation on Thr-309 and Ser-474 is required for full activity.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.

Target information above from: UniProt accessionP31751 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

References for AKT2 protein (ab42593)

ab42593 has not yet been referenced specifically in any publications.

Publishing research using ab42593? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"