Overview

  • Product name
    Anti-AKT3 + AKT1 antibody [E45]
    See all AKT3+AKT1 primary antibodies
  • Description
    Rabbit monoclonal [E45] to AKT3 + AKT1
  • Specificity
    ab32038 detects Akt1 and Akt3.
  • Tested applications
    Suitable for: WB, IHC-P, ICC, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human AKT1 aa 450-550.
    Database link: P31749

  • Positive control
    • Squamous lung carcinoma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab32038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 19679212
IHC-P 1/250 - 1/1000.
ICC 1/1000.
IP 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    Images

    • Immunohistochemistry (Paraffin-embedded sections) analysis using ab32038 at a dilution of 1/1000 - 1/1250 and squamous lung carcinoma
    • All lanes : Anti-AKT3 + AKT1 antibody [E45] (ab32038) at 1/10000 dilution

      Lane 1 : GFP tagged AKT1 transfected 293T cell lysate
      Lane 2 : Empty vector transfected 293T cell lysate
      Lane 3 : GFP tagged AKT2 transfected 293T cell lysate
      Lane 4 : Empty vector transfected 293T cell lysate
      Lane 5 : GFP tagged AKT3 transfected 293T cell lysate
      Lane 6 : Empty vector transfected 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Exposure time : 1 second

      Blocking/Diluting buffer: 5% NFDM/TBST.

      Observed band: 82kDa, 56kDa.

      82kDa band represents GFP tagged AKT. 56kDa band represents endogenous AKT. 

    References

    This product has been referenced in:
    • Liao P  et al. Palmitoylated SCP1 is targeted to the plasma membrane and negatively regulates angiogenesis. Elife 6:N/A (2017). WB . Read more (PubMed: 28440748) »
    • Wang Y  et al. Intrinsic cellular signaling mechanisms determine the sensitivity of cancer cells to virus-induced apoptosis. Sci Rep 6:37213 (2016). Read more (PubMed: 27849011) »

    See all 7 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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