 image](#AKT3-Primary-antibodies-ab13919-1.jpg)
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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
Overview
Product name
Anti-AKT3 antibody [66C1247]
See all AKT3 products (11) ...
Description
Mouse monoclonal [66C1247] to AKT3
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: CSPTSQIDNIGEEEMDAS, corresponding to amino acids 119-136 of Human AKT3. This sequence is identical in human, mouse and rat.
CSPTSQIDNI GEEEMDAS
Positive control
Human kidney lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.1mg/ml BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
66C1247
Isotype
IgG1
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> AKT
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
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Immunocytochemistry/ Immunofluorescence-AKT3 antibody [66C1247](ab13919)
 image](/ps/datasheet/images/13/ab13919/AKT3-Primary-antibodies-ab13919-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab13919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml.Predicted molecular weight: 56 kDa.(Ab13919 can be used for detection of AKT3 in human, mouse and rat by western blot analysis.)
ICC/IF: Use a concentration of 1 - 5 µg/ml.
Target
Function
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
Tissue specificity
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Domain
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
Post-translational
modifications
Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
Cellular localization
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
Target information above from: UniProt accessionQ9Y243
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Akt3 antibody
- AKT3 kinase antibody
- AKT3_HUMAN antibody
see all
Database links
- Entrez Gene: 10000 Human
- Entrez Gene: 23797 Mouse
- Entrez Gene: 29414 Rat
- Omim: 611223 Human
- SwissProt: Q9Y243 Human
- SwissProt: Q9WUA6 Mouse
- SwissProt: Q63484 Rat
- Unigene: 498292 Human
see all
Anti-AKT3 antibody [66C1247] images:
Immunocytochemistry/ Immunofluorescence-AKT3 antibody [66C1247](ab13919)
ICC/IF image of ab13919 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13919, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
References for Anti-AKT3 antibody [66C1247] (ab13919)
This product has been referenced in:
- Shin SSet al. AKT2 is a downstream target of metabotropic glutamate receptor 1 (Grm1). Pigment Cell Melanoma Res 23:103-11 (2010).Read more (PubMed: 19843246) »
See 1 publication for this product
Publishing research using ab13919? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"