Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
Overview
Product name
Anti-AKT3 antibody
See all AKT3 products (11) ...
Description
Goat polyclonal to AKT3
Specificity
This antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT3 portein. This antibody does not cross-react with human AKT1/2.
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide: (C)SPTSQIDNIGEEEM, corresponding to Internal sequence amino acids 120-133 of Human AKT3
(C)SPTSQID NIGEEEM
Positive control
Human Hepatoblastoma HepG2 lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> AKT
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
-
Western blot - AKT3 antibody (ab24370)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 3 µg/ml.Detects a band of approximately 55 kDa (predicted molecular weight: 56 kDa).
Target
Function
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
Tissue specificity
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Domain
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
Post-translational
modifications
Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
Cellular localization
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
Target information above from: UniProt accessionQ9Y243
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Akt3 antibody
- AKT3 kinase antibody
- AKT3_HUMAN antibody
see all
Database links
- Entrez Gene: 10000 Human
- Entrez Gene: 23797 Mouse
- Entrez Gene: 29414 Rat
- Omim: 611223 Human
- SwissProt: Q9Y243 Human
- SwissProt: Q9WUA6 Mouse
- SwissProt: Q63484 Rat
- Unigene: 498292 Human
see all
Anti-AKT3 antibody images:
Western blot - AKT3 antibody (ab24370)
Anti-AKT3 antibody (ab24370) at 1 µg/ml + HepG2 lysate (35µg protein in RIPA buffer). at 35 µg
developed using the ECL technique
Predicted band size : 56 kDa
Observed band size : 50-55 kDa (why is the actual band size different from the predicted?)
References for Anti-AKT3 antibody (ab24370)
ab24370 has not yet been referenced specifically in any publications.
Publishing research using ab24370? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"