Overview

  • Product nameAnti-AKT3 antibodySee all AKT3 primary antibodies ...
  • Description
    Rabbit polyclonal to AKT3
  • SpecificityCrossreactivity of this antibody with Akt2 has been reported. Does not crossreact with Akt1.
  • Tested applicationsELISA, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide.

  • Positive control
    • Lovo, HeLa or 3T3 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab5922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.1 - 1 µg/ml.
IP Use 3-5µg for 106 cells.
WB Use at an assay dependent dilution. Predicted molecular weight: 56 kDa.

Target

  • FunctionIGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
  • Tissue specificityIn adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
  • Post-translational
    modifications
    Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Akt3 antibody
    • AKT3 kinase antibody
    • AKT3_HUMAN antibody
    • DKFZP434N0250 antibody
    • MPPH antibody
    • PKB Gamma antibody
    • PKBG antibody
    • PRKBG antibody
    • Protein kinase Akt-3 antibody
    • Protein Kinase AKT3 antibody
    • Protein Kinase B Gamma antibody
    • RAC gamma antibody
    • RAC Gamma Serine/Threonine Protein Kinase antibody
    • RAC-gamma serine/threonine-protein kinase antibody
    • RAC-PK-gamma antibody
    • RACPK Gamma antibody
    • Serine threonine protein kinase Akt 3 antibody
    • Serine Threonine Protein Kinase AKT3 antibody
    • STK 2 antibody
    • STK-2 antibody
    • STK2 antibody
    • V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody
    • V akt murine thymoma viral oncogene homolog 3 antibody
    • V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma antibody
    • VAKT Murine Thymoma Viral Oncogene Homolog 3 antibody
    see all

Anti-AKT3 antibody images

  • All lanes : Anti-AKT3 antibody (ab5922) at 0.5 µg/ml

    Lane 1 : Lovo cell lysates.
    Lane 2 : Lovo cell lysates with Anti AKT3 peptide


    Predicted band size : 56 kDa

References for Anti-AKT3 antibody (ab5922)

This product has been referenced in:
  • Piao Y  et al. Acquired resistance to anti-VEGF therapy in glioblastoma is associated with a mesenchymal transition. Clin Cancer Res 19:4392-403 (2013). Mouse . Read more (PubMed: 23804423) »

See 1 Publication for this product

Product Wall

It is specific, no cross reactivity with AKT1 and AKT2 has been observed.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"