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Product Name
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AKT3 antibody
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See all AKT3 antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to AKT3
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Immunogen
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Synthetic peptide.
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at a concentration of 0.1 - 1.0 µg/ml.
IP: Use 3-5µg for 106 cells.
WB: Use at an assay dependent dilution. Predicted molecular weight: 56 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Lovo, HeLa or 3T3 whole cell lysate.
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Cellular localization
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Cytoplasmic and membrane associated after cell stimulation leading to its translocation.
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Research areas
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Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> AKT
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
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Relevance
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Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphtidylinositol 3 kinase (PI 3 kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI3 kinase and Akt/PKB kinase activity. There are 3 known isoforms of this enzyme in mammalian cells (1/alpha, 2/beta and 3/gamma). IGF1 leads to the activation of AKT3, which may play a role in regulating cell survival. It is capable of phosphorylating several known proteins.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AKT3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AKT3 antibody images, references, products related to ab5922 and other tools.
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AKT3 antibody images:
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 - AKT3 antibody (ab5922)
Western blot analysis of AKT3 protein on LOVO cell lysates. A: purified Anti-AKT3; B: Anti-AKT3 with blocking peptide.
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Search PubMed (MEDLINE) for references to AKT3
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AKT3 antibody - more information
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Customer reviews (feedback) regarding AKT3 antibody |
Customer FAQs regarding AKT3 antibody |
Protocols for AKT3 antibody |
Price and availability of products related to AKT3 antibody |
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AKT3 antibody - related products:
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AKT3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about AKT3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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