Anti-AKT3 antibody (ab5922)

Overview

• Product nameAnti-AKT3 antibodySee all AKT3 primary antibodies ...
• Description
  Rabbit polyclonal to AKT3
• SpecificityCrossreactivity of this antibody with Akt2 has been reported. Does not crossreact with Akt1.
• Tested applicationsELISA, IP, WB more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide.

• Positive controlLovo, HeLa or 3T3 whole cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Signal transduction
  • Protein phosphorylation
  • Serine/threonine kinases
  • AKT

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • PKB / AKT

Applications

Target

• FunctionIGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
• Tissue specificityIn adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
• Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
  Contains 1 AGC-kinase C-terminal domain.
  Contains 1 PH domain.
  Contains 1 protein kinase domain.
• DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
• Post-translational
  modificationsPhosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
• Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.

Target information above from: UniProt accession Q9Y243 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10000 Human
  • Entrez Gene: 10000 Human
  • Entrez Gene: 10000 Human
  • Entrez Gene: 23797 Mouse
  • Entrez Gene: 29414 Rat
  • Omim: 611223 Human
  • SwissProt: Q9Y243 Human
  • SwissProt: Q9Y243 Human

  • SwissProt: Q9Y243 Human
  • SwissProt: Q9WUA6 Mouse
  • SwissProt: Q63484 Rat
  • Unigene: 498292 Human
  • Unigene: 235194 Mouse
  • Unigene: 10506 Rat

  see all

• Alternative names
  Akt3 antibodyAKT3 kinase antibodyAKT3_HUMAN antibody

  DKFZP434N0250 antibodyPKB Gamma antibodyPKBG antibodyPRKBG antibodyProtein kinase Akt-3 antibodyProtein Kinase AKT3 antibodyProtein Kinase B Gamma antibodyRAC gamma antibodyRAC Gamma Serine/Threonine Protein Kinase antibodyRAC-gamma serine/threonine-protein kinase antibodyRAC-PK-gamma antibodyRACPK Gamma antibodySerine threonine protein kinase Akt 3 antibodySerine Threonine Protein Kinase AKT3 antibodySTK 2 antibodySTK-2 antibodySTK2 antibodyV akt murine thymoma viral oncogene homolog 3 protein kinase B gamma antibodyVAKT Murine Thymoma Viral Oncogene Homolog 3 antibody

  see all

Anti-AKT3 antibody images

• 

  Western blot - AKT3 antibody (ab5922)
  All lanes : Anti-AKT3 antibody (ab5922) at 0.5 µg/ml
  
  Lane 1 : Lovo cell lysates.
  Lane 2 : Lovo cell lysates with Anti AKT3 peptide
  
  
  Predicted band size : 56 kDa