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Overview

  • Product nameAnti-AKT3 antibodySee all AKT3 primary antibodies ...
  • Description
    Rabbit polyclonal to AKT3
  • SpecificityCrossreactivity of this antibody with Akt2 has been reported. Does not crossreact with Akt1.
  • Tested applicationsELISA, IP, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide.

  • Positive controlLovo, HeLa or 3T3 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab5922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: Use a concentration of 0.1 - 1 µg/ml.
IP IP: Use 3-5µg for 106 cells.
WB WB: Use at an assay dependent dilution. Predicted molecular weight: 56 kDa.

Target

  • FunctionIGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
  • Tissue specificityIn adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
  • Post-translational
    modifications
    Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Target information above from: UniProt accession Q9Y243 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
      Akt3 antibodyAKT3 kinase antibodyAKT3_HUMAN antibody
      DKFZP434N0250 antibodyPKB Gamma antibodyPKBG antibodyPRKBG antibodyProtein kinase Akt-3 antibodyProtein Kinase AKT3 antibodyProtein Kinase B Gamma antibodyRAC gamma antibodyRAC Gamma Serine/Threonine Protein Kinase antibodyRAC-gamma serine/threonine-protein kinase antibodyRAC-PK-gamma antibodyRACPK Gamma antibodySerine threonine protein kinase Akt 3 antibodySerine Threonine Protein Kinase AKT3 antibodySTK 2 antibodySTK-2 antibodySTK2 antibodyV akt murine thymoma viral oncogene homolog 3 protein kinase B gamma antibodyVAKT Murine Thymoma Viral Oncogene Homolog 3 antibody
    see all

Anti-AKT3 antibody images

  • All lanes : Anti-AKT3 antibody (ab5922) at 0.5 µg/ml

    Lane 1 : Lovo cell lysates.
    Lane 2 : Lovo cell lysates with Anti AKT3 peptide


    Predicted band size : 56 kDa

References for Anti-AKT3 antibody (ab5922)

ab5922 has not yet been referenced specifically in any publications.

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It is specific, no cross reactivity with AKT1 and AKT2 has been observed.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"