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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
Overview
Product name
Anti-AKT3 antibody
See all AKT3 products (11) ...
Description
Rabbit polyclonal to AKT3
Specificity
Crossreactivity of this antibody with Akt2 has been reported. Does not crossreact with Akt1.
Tested applications
ELISA, IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide.
Positive control
Lovo, HeLa or 3T3 whole cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> AKT
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
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Western blot - AKT3 antibody (ab5922)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use a concentration of 0.1 - 1.0 µg/ml.
IP: Use 3-5µg for 106 cells.
WB: Use at an assay dependent dilution. Predicted molecular weight: 56 kDa.
Target
Function
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
Tissue specificity
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Domain
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
Post-translational
modifications
Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
Cellular localization
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
Target information above from: UniProt accessionQ9Y243
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Akt3 antibody
- AKT3 kinase antibody
- AKT3_HUMAN antibody
see all
Database links
- Entrez Gene: 10000 Human
- Entrez Gene: 23797 Mouse
- Entrez Gene: 29414 Rat
- Omim: 611223 Human
- SwissProt: Q9Y243 Human
- SwissProt: Q9WUA6 Mouse
- SwissProt: Q63484 Rat
- Unigene: 498292 Human
see all
Anti-AKT3 antibody images:
Western blot - AKT3 antibody (ab5922)
All lanes : Anti-AKT3 antibody (ab5922) at 0.5 µg/ml
Lane 1 : Lovo cell lysates.
Lane 2 : Lovo cell lysates with Anti AKT3 peptide
Predicted band size : 56 kDa
References for Anti-AKT3 antibody (ab5922)
ab5922 has not yet been referenced specifically in any publications.
Publishing research using ab5922? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"