Overview

  • Product nameAnti-AKT3 antibodySee all AKT3 primary antibodies ...
  • Description
    Rabbit polyclonal to AKT3
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human AKT3.

    (Peptide available as ab89371.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: HEK293 whole cell; HUVEC whole cell; Human brain tissue; Human lung tissue; Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab72177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).Can be blocked with Human AKT3 peptide (ab89371).

Target

  • FunctionIGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
  • Tissue specificityIn adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
  • Post-translational
    modifications
    Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Akt3 antibody
    • AKT3 kinase antibody
    • AKT3_HUMAN antibody
    • DKFZP434N0250 antibody
    • MPPH antibody
    • PKB Gamma antibody
    • PKBG antibody
    • PRKBG antibody
    • Protein kinase Akt-3 antibody
    • Protein Kinase AKT3 antibody
    • Protein Kinase B Gamma antibody
    • RAC gamma antibody
    • RAC Gamma Serine/Threonine Protein Kinase antibody
    • RAC-gamma serine/threonine-protein kinase antibody
    • RAC-PK-gamma antibody
    • RACPK Gamma antibody
    • Serine threonine protein kinase Akt 3 antibody
    • Serine Threonine Protein Kinase AKT3 antibody
    • STK 2 antibody
    • STK-2 antibody
    • STK2 antibody
    • V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody
    • V akt murine thymoma viral oncogene homolog 3 antibody
    • V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma antibody
    • VAKT Murine Thymoma Viral Oncogene Homolog 3 antibody
    see all

Anti-AKT3 antibody images

  • All lanes : Anti-AKT3 antibody (ab72177) at 1 µg/ml

    Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
    Lane 2 : Lung (Human) Tissue Lysate
    Lane 3 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes

References for Anti-AKT3 antibody (ab72177)

ab72177 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"