Anti-AKT3 antibody (ab72177)
- Product nameAnti-AKT3 antibodySee all AKT3 primary antibodies ...
- DescriptionRabbit polyclonal to AKT3
- Tested applicationsWB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human AKT3.
(Peptide available as ab89371.)
- Positive control
- This antibody gave a positive signal in the following lysates: HEK293 whole cell; HUVEC whole cell; Human brain tissue; Human lung tissue; Human kidney tissue.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab72177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).Can be blocked with AKT3 peptide (ab89371).|
- FunctionIGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
- Tissue specificityIn adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
- Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
- DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
modificationsPhosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
- Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
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- RAC Gamma Serine/Threonine Protein Kinase antibody
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- RAC-PK-gamma antibody
- RACPK Gamma antibody
- Serine threonine protein kinase Akt 3 antibody
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- STK 2 antibody
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Anti-AKT3 antibody images
All lanes : Anti-AKT3 antibody (ab72177) at 1 µg/ml
Lane 1 :
Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Lung (Human) Tissue Lysate
Lane 3 :
Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 55 kDa
Observed band size : 55 kDa
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 minutes
References for Anti-AKT3 antibody (ab72177)
ab72177 has not yet been referenced specifically in any publications.