Anti-AKT3 antibody (ab89430)

Overview

• Product nameAnti-AKT3 antibodySee all AKT3 primary antibodies ...
• Description
  Mouse monoclonal to AKT3
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Recombinant fragment (with 26 kDa tag): EAIQAVADRL QRQEEERMNC SPTSQIDNIG EEEMDASTTH HKRKTMNDFD YLKLLGKGTF GKVILVREKA SGKYYAMKIL KKEVIIAKDE , corresponding to amino acids 100-189 of Human AKT3 (AAD29089) (sequence does not include tag)

  Run BLAST with Run BLAST with
• Positive controlAKT3 recombinant protein, HeLa cell lysate, Raw 264.7 cell lysate, MCF7 cell lysate and NIH/3T3 cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• IsotypeIgG2a
• Research Areas
  • Cancer
  • Signal transduction
  • Protein phosphorylation
  • Serine/threonine kinases
  • AKT

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • PKB / AKT

Applications

Target

• FunctionIGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
• Tissue specificityIn adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
• Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
  Contains 1 AGC-kinase C-terminal domain.
  Contains 1 PH domain.
  Contains 1 protein kinase domain.
• DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
• Post-translational
  modificationsPhosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
• Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.

Target information above from: UniProt accession Q9Y243 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10000 Human
  • Entrez Gene: 10000 Human
  • Entrez Gene: 10000 Human
  • Entrez Gene: 23797 Mouse
  • Omim: 611223 Human
  • SwissProt: Q9Y243 Human
  • SwissProt: Q9Y243 Human
  • SwissProt: Q9Y243 Human

  • SwissProt: Q9WUA6 Mouse
  • Unigene: 498292 Human
  • Unigene: 235194 Mouse

  see all

• Alternative names
  Akt3 antibodyAKT3 kinase antibodyAKT3_HUMAN antibody

  DKFZp434N0250 antibodyPKB gamma antibodyPKBG antibodyPRKBG antibodyProtein kinase Akt-3 antibodyProtein Kinase AKT3 antibodyProtein kinase B gamma antibodyRAC gamma antibodyRAC gamma serine/threonine protein kinase antibodyRAC-gamma serine/threonine-protein kinase antibodyRAC-PK-gamma antibodyRACPK Gamma antibodySerine threonine protein kinase Akt 3 antibodySerine threonine protein kinase Akt3 antibodySTK 2 antibodySTK-2 antibodySTK2 antibodyV akt murine thymoma viral oncogene homolog 3 protein kinase B gamma antibody

  see all

Anti-AKT3 antibody images

• 

  Western blot - AKT3 antibody (ab89430)
  Anti-AKT3 antibody (ab89430) at 5 µg/ml + AKT3 recombinant protein fragment at 0.2 µg
  
  Predicted band size : 56 kDa
  Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  
• 

  Western blot - AKT3 antibody (ab89430)
  Anti-AKT3 antibody (ab89430) at 5 µg/ml + HeLa cell lysate at 50 µg
  
  Predicted band size : 56 kDa
  Observed band size : 56 kDa
  
• 

  Western blot - AKT3 antibody (ab89430)
  Anti-AKT3 antibody (ab89430) at 5 µg/ml + Raw 264.7 cell lysate at 50 µg
  
  Predicted band size : 56 kDa
  Observed band size : 56 kDa
  
• 

  Western blot - AKT3 antibody (ab89430)
  Anti-AKT3 antibody (ab89430) at 5 µg/ml + NIH/3T3 cell lysate at 50 µg
  
  Predicted band size : 56 kDa
  Observed band size : 56 kDa
  
• 

  ELISA - AKT3 antibody (ab89430)
  Detection limit for recombinant AKT3 is approximately 0.1ng/ml as a capture antibody.
• 

  Western blot - AKT3 antibody (ab89430)
  Anti-AKT3 antibody (ab89430) at 5 µg/ml + MCF7 cell lysate at 50 µg
  
  Predicted band size : 56 kDa