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Anti-AKT3 antibody (ab95218)

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Overview

Product name

Anti-AKT3 antibody
See all AKT3 products (11) ...

Description

Rabbit polyclonal to AKT3

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Rrecombinant full length Human AKT3 protein (UniProt ID: Q9Y243).

Positive control

HL60 cell line lysates

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab95218 is purified through a protein A column, followed by protein affinity purification.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - AKT3 antibody (ab95218)Western blot - AKT3 antibody (ab95218) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab95218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.

Tissue specificity

In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.

Domain

Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.

Post-translational
modifications

Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.

Cellular localization

Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.

Target information above from: UniProt accessionQ9Y243 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Akt3 antibody
  • AKT3 kinase antibody
  • AKT3_HUMAN antibody
  • DKFZp434N0250 antibody
  • PKB gamma antibody
  • PKBG antibody
  • PRKBG antibody
  • Protein kinase Akt-3 antibody
  • Protein Kinase AKT3 antibody
  • Protein kinase B gamma antibody
  • RAC gamma antibody
  • RAC gamma serine/threonine protein kinase antibody
  • RAC-gamma serine/threonine-protein kinase antibody
  • RAC-PK-gamma antibody
  • RACPK Gamma antibody
  • Serine threonine protein kinase Akt 3 antibody
  • Serine threonine protein kinase Akt3 antibody
  • STK 2 antibody
  • STK-2 antibody
  • STK2 antibody
  • V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma antibody
see all

Anti-AKT3 antibody images:

  Western blot - AKT3 antibody (ab95218)

Western blot - AKT3 antibody (ab95218)

Anti-AKT3 antibody (ab95218) at 1/100 dilution + HL60 cell line lysates at 35 µg

Predicted band size : 56 kDa

References for Anti-AKT3 antibody (ab95218)

ab95218 has not yet been referenced specifically in any publications.

Publishing research using ab95218? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"