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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
Overview
Product name
AKT3 protein (Active)
See all AKT3 products (4) ...
Protein description
Recombinant full length human AKT3 (amino acids 2-479) with N-terminal His tag expressed in a baculovirus expression system, 60 kDa (SwissProt ID Q9Y243).
Properties
Purity
>95% by SDS-PAGE
Biological activity
Specific Activity: 10 pmol/min/µg.
Form
Liquid
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10% Glycerol, 0.05% Tween 20, 25mM Tris HCl, 138mM Sodium chloride, pH 8
Concentration
Concentration information loading...
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> AKT
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
Applications
Show applications key
Our Abpromise guarantee covers the use of ab79799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
Kinase Assay: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
Tissue specificity
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Domain
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
Post-translational
modifications
Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
Cellular localization
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
Target information above from: UniProt accessionQ9Y243
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
AKT3 protein (Active) images:
SDS-PAGE - AKT3 protein (Active) (ab79799)
10% SDS-PAGE, Coomassie staining. Lane 1: ab79799 5µg.Lane 2: Protein marker.
References for AKT3 protein (Active) (ab79799)
ab79799 has not yet been referenced specifically in any publications.
Publishing research using ab79799? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"