Overview

  • Product nameAnti-ALDH1A1 antibody [EP1933Y]
    See all ALDH1A1 primary antibodies
  • Description
    Rabbit monoclonal [EP1933Y] to ALDH1A1
  • Tested applicationsICC/IF, WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of human Aldh1a1

  • Positive control
    • HepG2 cell lysate; human liver tissue
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab52492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21396117
WB 1/500 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IP 1/30.
ICC 1/100 - 1/250.
Flow Cyt 1/1000.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionBinds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
  • PathwayCofactor metabolism; retinol metabolism.
  • Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetaldehyde dehydrogenase 1 antibody
    • AHD2 antibody
    • AL1A1_HUMAN antibody
    • ALDC antibody
    • Aldehyde dehydrogenase 1 family member A1 antibody
    • Aldehyde dehydrogenase 1 soluble antibody
    • Aldehyde dehydrogenase 1A1 antibody
    • Aldehyde Dehydrogenase antibody
    • Aldehyde dehydrogenase cytosolic antibody
    • Aldehyde dehydrogenase family 1 member A1 antibody
    • Aldehyde dehydrogenase liver cytosolic antibody
    • ALDH 1 antibody
    • ALDH 1A1 antibody
    • ALDH class 1 antibody
    • ALDH, liver cytosolic antibody
    • ALDH-E1 antibody
    • ALDH1 A1 antibody
    • ALDH1 antibody
    • ALDH11 antibody
    • ALDH1A1 antibody
    • ALHDII antibody
    • cytosolic antibody
    • epididymis luminal protein 12 antibody
    • epididymis luminal protein 9 antibody
    • epididymis secretory sperm binding protein Li 53e antibody
    • HEL-S-53e antibody
    • MGC2318 antibody
    • PUMB1 antibody
    • RALDH 1 antibody
    • RalDH1 antibody
    • Retinal dehydrogenase 1 antibody
    see all

Anti-ALDH1A1 antibody [EP1933Y] images

  • Anti-ALDH1A1 antibody [EP1933Y] (ab52492) at 1/2000 dilution + HepG2 cell lysate at 10 µg

    Secondary
    HRP labeled Goat anti-rabbit at 1/2000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • Immunohistochemical analysis of paraffin-embedded human liver using ab52492 at 1/100 dilution.
  • ICC/IF image of ab52492 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52492, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab52492 showing positive staining in Normal brain tissue.

  • ab52492 showing positive staining in Normal lung tissue.

  • ab52492 showing positive staining in Normal kidney tissue.

  • Overlay histogram showing HepG2 cells stained with ab52492 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52492, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-ALDH1A1 antibody [EP1933Y] (ab52492)

This product has been referenced in:
  • Ferrand N  et al. Loss of WISP2/CCN5 in estrogen-dependent MCF7 human breast cancer cells promotes a stem-like cell phenotype. PLoS One 9:e87878 (2014). WB ; Human . Read more (PubMed: 24498388) »
  • DA Cruz Paula A  et al. Co-expression of stem cell markers ALDH1 and CD44 in non-malignant and neoplastic lesions of the breast. Anticancer Res 34:1427-34 (2014). IHC-P ; Human . Read more (PubMed: 24596390) »

See all 19 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12% acrylamide gel)
Sample Human Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 24 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (fibroblast)
Loading amount 30 µg
Specification fibroblast
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Username

Marta Canamero

Verified customer

Submitted Aug 21 2008

Thank you for submitting an Abreview for ab52492. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested application, we would like to follow up on this t...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (liver)
Specification liver
Fixation Paraformaldehyde
Permeabilization Yes - Flow Cyt Perm/Wash BufferI R&D FC005
Username

Abcam user community

Verified customer

Submitted Sep 12 2012

Vielen Dank für Ihren Anruf.

Ich freue mich Ihnen nun bestätigen zu können, dass die Konzentration 0.130 mg/mL ist. (von Lot 1315178).

Ich würde Sie außerdem noch gerne darauf hinweisen, dass ich für e...

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Thank you for contacting Abcam.

Based on the information we have for ab52492, we would recommend that you use heat mediated antigen retrieval with sodium citrate. I have attached our Abcams protocol booklet that contains information about thi...

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Thank you for contacting Abcam.



The immunogen shares 88% identity with canine Aldh1a1.

Please let me know if there is anything else I can help you with.

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (tumor xenograft in mouse)
Specification tumor xenograft in mouse
Fixative Methanol
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Feb 23 2012

Thank you for contacting Abcam regarding ab52492.


I am sorry that you are experiencing some difficulties detecting the correct molecular weight band in WB with this antibody. I have reviewed the protocol information you provided and I a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"