Anti-ALDH1A1 antibody [EP1933Y] (ab52492)

Overview

• Product nameAnti-ALDH1A1 antibody [EP1933Y]See all ALDH1A1 primary antibodies ...
• Description
  Rabbit monoclonal [EP1933Y] to ALDH1A1
• Tested applicationsICC/IF, WB, IP, ICC, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues near the C-terminus of human Aldh1a1

• Positive controlHepG2 cell lysate; human liver tissue
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP1933Y
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Hematopoietic Progenitors
  • Intracellular Molecules

  • Cancer
  • Tumor biomarkers
  • Other

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Parkinson's disease
  • Other

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Soma marker

  • Neuroscience
  • Neurology process
  • Neurogenesis

Applications

Target

• FunctionBinds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
• PathwayCofactor metabolism; retinol metabolism.
• Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession P00352 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 216 Human
  • Entrez Gene: 11668 Mouse
  • Entrez Gene: 24188 Rat
  • Omim: 100640 Human
  • SwissProt: P00352 Human
  • SwissProt: P24549 Mouse
  • SwissProt: P51647 Rat
  • Unigene: 76392 Human

  • Unigene: 250866 Mouse
  • Unigene: 6132 Rat

  see all

• Alternative names
  Acetaldehyde dehydrogenase 1 antibodyAHD2 antibodyAL1A1_HUMAN antibody

  ALDC antibodyAldehyde dehydrogenase 1 family member A1 antibodyAldehyde dehydrogenase 1 soluble antibodyAldehyde dehydrogenase 1A1 antibodyAldehyde Dehydrogenase antibodyAldehyde dehydrogenase cytosolic antibodyAldehyde dehydrogenase family 1 member A1 antibodyAldehyde dehydrogenase liver cytosolic antibodyALDH 1 antibodyALDH 1A1 antibodyALDH class 1 antibodyALDH-E1 antibodyALDH1 A1 antibodyALDH1 antibodyALDH11 antibodyALDH1A1 antibodyALHDII antibodycytosolic antibodyMGC2318 antibodyPUMB1 antibodyRALDH 1 antibodyRalDH1 antibodyRetinal dehydrogenase 1 antibody

  see all

Anti-ALDH1A1 antibody [EP1933Y] images

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  Western blot - ALDH1A1 antibody [EP1933Y] (ab52492)
  Anti-ALDH1A1 antibody [EP1933Y] (ab52492) at 1/2000 dilution + HepG2 cell lysate at 10 µg
  
  Secondary
  HRP labeled Goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 55 kDa
  Observed band size : 55 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ALDH1A1 antibody [EP1933Y] (ab52492)
  Immunohistochemical analysis of paraffin-embedded human liver using ab52492 at 1/100 dilution.
• 

  Immunocytochemistry/ Immunofluorescence - ALDH1A1 antibody [EP1933Y] (ab52492)
  ICC/IF image of ab52492 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52492, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-ALDH1A1 antibody [EP1933Y](ab52492)
  ab52492 showing positive staining in Normal brain tissue.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-ALDH1A1 antibody [EP1933Y](ab52492)
  ab52492 showing positive staining in Normal lung tissue.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-ALDH1A1 antibody [EP1933Y](ab52492)
  ab52492 showing positive staining in Normal kidney tissue.