Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab52492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21396117
WB 1/500 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IP 1/30.
ICC 1/100 - 1/250.
Flow Cyt 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionBinds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
  • PathwayCofactor metabolism; retinol metabolism.
  • Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetaldehyde dehydrogenase 1 antibody
    • AHD2 antibody
    • AL1A1_HUMAN antibody
    • ALDC antibody
    • Aldehyde dehydrogenase 1 family member A1 antibody
    • Aldehyde dehydrogenase 1 soluble antibody
    • Aldehyde dehydrogenase 1A1 antibody
    • Aldehyde Dehydrogenase antibody
    • Aldehyde dehydrogenase cytosolic antibody
    • Aldehyde dehydrogenase family 1 member A1 antibody
    • Aldehyde dehydrogenase liver cytosolic antibody
    • ALDH 1 antibody
    • ALDH 1A1 antibody
    • ALDH class 1 antibody
    • ALDH, liver cytosolic antibody
    • ALDH-E1 antibody
    • ALDH1 A1 antibody
    • ALDH1 antibody
    • ALDH11 antibody
    • ALDH1A1 antibody
    • ALHDII antibody
    • cytosolic antibody
    • epididymis luminal protein 12 antibody
    • epididymis luminal protein 9 antibody
    • epididymis secretory sperm binding protein Li 53e antibody
    • HEL-S-53e antibody
    • MGC2318 antibody
    • PUMB1 antibody
    • RALDH 1 antibody
    • RalDH1 antibody
    • Retinal dehydrogenase 1 antibody
    see all

Anti-ALDH1A1 antibody [EP1933Y] images

  • Anti-ALDH1A1 antibody [EP1933Y] (ab52492) at 1/2000 dilution + HepG2 cell lysate at 10 µg

    Secondary
    HRP labeled Goat anti-rabbit at 1/2000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • Immunohistochemical analysis of paraffin-embedded human liver using ab52492 at 1/100 dilution.
  • ICC/IF image of ab52492 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52492, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab52492 showing positive staining in Normal brain tissue.

  • ab52492 showing positive staining in Normal lung tissue.

  • ab52492 showing positive staining in Normal kidney tissue.

  • Overlay histogram showing HepG2 cells stained with ab52492 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52492, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-ALDH1A1 antibody [EP1933Y] (ab52492)

This product has been referenced in:

See all 22 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12% acrylamide gel)
Sample Human Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted Sep 24 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (fibroblast)
Loading amount 30 µg
Specification fibroblast
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Mar 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Username

Marta Canamero

Verified customer

Submitted Aug 21 2008

Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10%)
Loading amount 15 µg
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
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Submitted Aug 19 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10% Tris-Glycine)
Sample Mouse Cell lysate - whole cell (Neural progenitors)
Specification Neural progenitors
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Feb 26 2015

Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (10% Tris-Glycine)
Sample Cow Cell lysate - whole cell (MDBK cells)
Specification MDBK cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Feb 16 2015

Application Western blot
Loading amount 75 µg
Gel Running Conditions Reduced Denaturing (10% Tris-Glycine)
Sample Human Cell lysate - whole cell (HEK-293T)
Specification HEK-293T
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Feb 11 2015

Thank you for submitting an Abreview for ab52492. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested application, we would like to follow up on this t...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (liver)
Specification liver
Fixation Paraformaldehyde
Permeabilization Yes - Flow Cyt Perm/Wash BufferI R&D FC005
Username

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Verified customer

Submitted Sep 12 2012

Vielen Dank für Ihren Anruf.

Ich freue mich Ihnen nun bestätigen zu können, dass die Konzentration 0.130 mg/mL ist. (von Lot 1315178).

Ich würde Sie außerdem noch gerne darauf hinweisen, dass ich für e...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"