Overview

  • Product nameAnti-ALDH1A1 antibody [EP1933Y]See all ALDH1A1 primary antibodies ...
  • Description
    Rabbit monoclonal [EP1933Y] to ALDH1A1
  • Tested applicationsICC/IF, WB, IP, ICC, Flow Cyt, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of human Aldh1a1

  • Positive control
    • HepG2 cell lysate; human liver tissue
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab52492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21396117
WB 1/500 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IP 1/30.
ICC 1/100 - 1/250.
Flow Cyt 1/1000.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionBinds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
  • PathwayCofactor metabolism; retinol metabolism.
  • Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
  • Cellular localizationCytoplasm.
  • Target information above from: UniProt accession P00352 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Acetaldehyde dehydrogenase 1 antibody
    • AHD2 antibody
    • AL1A1_HUMAN antibody
    • ALDC antibody
    • Aldehyde dehydrogenase 1 family member A1 antibody
    • Aldehyde dehydrogenase 1 soluble antibody
    • Aldehyde dehydrogenase 1A1 antibody
    • Aldehyde Dehydrogenase antibody
    • Aldehyde dehydrogenase cytosolic antibody
    • Aldehyde dehydrogenase family 1 member A1 antibody
    • Aldehyde dehydrogenase liver cytosolic antibody
    • ALDH 1 antibody
    • ALDH 1A1 antibody
    • ALDH class 1 antibody
    • ALDH-E1 antibody
    • ALDH1 A1 antibody
    • ALDH1 antibody
    • ALDH11 antibody
    • ALDH1A1 antibody
    • ALHDII antibody
    • cytosolic antibody
    • MGC2318 antibody
    • PUMB1 antibody
    • RALDH 1 antibody
    • RalDH1 antibody
    • Retinal dehydrogenase 1 antibody
    see all

Anti-ALDH1A1 antibody [EP1933Y] images

  • Anti-ALDH1A1 antibody [EP1933Y] (ab52492) at 1/2000 dilution + HepG2 cell lysate at 10 µg

    Secondary
    HRP labeled Goat anti-rabbit at 1/2000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • Immunohistochemical analysis of paraffin-embedded human liver using ab52492 at 1/100 dilution.
  • ICC/IF image of ab52492 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52492, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab52492 showing positive staining in Normal brain tissue.

  • ab52492 showing positive staining in Normal lung tissue.

  • ab52492 showing positive staining in Normal kidney tissue.

  • Overlay histogram showing HepG2 cells stained with ab52492 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52492, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-ALDH1A1 antibody [EP1933Y] (ab52492)

This product has been referenced in:
  • Ko SB  et al. Inflammation increases cells expressing ZSCAN4 and progenitor cell markers in the adult pancreas. Am J Physiol Gastrointest Liver Physiol 304:G1103-16 (2013). Read more (PubMed: 23599043) »
  • Gomez-Casal R  et al. Non-small cell lung cancer cells survived ionizing radiation treatment display cancer stem cell and epithelial-mesenchymal transition phenotypes. Mol Cancer 12:94 (2013). Read more (PubMed: 23947765) »

See all 13 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12% acrylamide gel)
Sample Human Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 24 2013

Thank you for submitting an Abreview for ab52492. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested application, we would like to follow up on this t...

Read More
Application Flow Cytometry
Sample Mouse Cell (liver)
Specification liver
Fixation Paraformaldehyde
Permeabilization Yes - Flow Cyt Perm/Wash BufferI R&D FC005
Username

Abcam user community

Verified customer

Submitted Sep 12 2012

Vielen Dank für Ihren Anruf.

Ich freue mich Ihnen nun bestätigen zu können, dass die Konzentration 0.130 mg/mL ist. (von Lot 1315178).

Ich würde Sie außerdem noch gerne darauf hinweisen, dass ich für e...

Read More

Thank you for contacting Abcam.

Based on the information we have for ab52492, we would recommend that you use heat mediated antigen retrieval with sodium citrate. I have attached our Abcams protocol booklet that contains information about thi...

Read More

Thank you for contacting Abcam.



The immunogen shares 88% identity with canine Aldh1a1.

Please let me know if there is anything else I can help you with.

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (tumor xenograft in mouse)
Specification tumor xenograft in mouse
Fixative Methanol
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Feb 23 2012

Thank you for contacting Abcam regarding ab52492.


I am sorry that you are experiencing some difficulties detecting the correct molecular weight band in WB with this antibody. I have reviewed the protocol information you provided and I a...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: autoclave
Permeabilization Yes - tritonx
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 14 2011

Thank you for your enquiry and your interest in our products. I can confirm that both antibodies recognize rat ALDH1A1, however the immunogens used to raise these antibodies were different. Whilst, for ab23375 a synthetic peptide conjugated to K...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"