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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Overview
Product name
Anti-ALDH1A1 antibody - Neuronal Marker
See all ALDH1A1 products (13) ...
Description
Rabbit polyclonal to ALDH1A1 - Neuronal Marker
Specificity
Rabbit polyclonal to ALDH1A1 (ab23375) detects ALDH1A1 at the predicted MW (54 kDa). The immunogen used to generate ab23375 will also detect ALDH1A7 [P13601] with 100% identity.
Tested applications
IHC-Fr, ICC/IF, IHC-FrFl, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Goat, Dog, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Rat ALDH1A1.
(Peptide available as ab25700.)
Positive control
This antibody gave a positive result in the following lysates: Mouse Brain Tissue, Mouse Liver Tissue, Rat Liver Tissue, Mouse Embryonic Brain (E14) Tissue and HepG2.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-Fr: 1/200 - 1/400 (See Abreview).
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
Pathway
Cofactor metabolism; retinol metabolism.
Sequence similarities
Belongs to the aldehyde dehydrogenase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP00352
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cytosolic antibody
- Acetaldehyde dehydrogenase 1 antibody
- AHD2 antibody
see all
Database links
- Entrez Gene: 216 Human
- Entrez Gene: 11668 Mouse
- Entrez Gene: 24188 Rat
- Omim: 100640 Human
- SwissProt: P00352 Human
- SwissProt: P24549 Mouse
- SwissProt: P51647 Rat
- SwissProt: P51977 Sheep
see all
Anti-ALDH1A1 antibody - Neuronal Marker images:
Immunocytochemistry/ Immunofluorescence - ALDH1A1 antibody - Neuronal Marker (ab23375)
ICC/IF image of ab23375 stained rat PC12 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab23375, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ALDH1A1 antibody - Neuronal Marker (ab23375)
IHC image of ALDH1A1 staining in rat brain FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23375, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
- ALDH1A1 antibody - Neuronal Marker (ab23375)
ab23375 staining ALDH1A1 in Mouse hypothalamic tanycytes by IHC-FrFl (Immunohistochemistry Free-floating). 40 µm free-floating sections were incubated with primary antibody (1/300) for 18 hours at 4°C in blocking solution. An Alexa Fluor® 555-conjugated Donkey polyclonal to rabbit IgG (1/300) was used as secondary antibody.
This image is courtesy of an anonymous Abreview
Western blot - ALDH1A1 antibody - Neuronal Marker (ab23375)
All lanes : Anti-ALDH1A1 antibody - Neuronal Marker (ab23375) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 54 kDa
Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 90 seconds
Western blot - ALDH1A1 antibody - Neuronal Marker (ab23375)
All lanes : Anti-ALDH1A1 antibody - Neuronal Marker (ab23375) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lane 4 : E14 Mouse Embryo Brain Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 51,54 kDa (why is the actual band size different from the predicted?)
Exposure time : 90 seconds
References for Anti-ALDH1A1 antibody - Neuronal Marker (ab23375)
This product has been referenced in:
- Wang Cet al. Multiple Retinol and Retinal Dehydrogenases Catalyze All-trans-retinoic Acid Biosynthesis in Astrocytes. J Biol Chem 286:6542-53 (2011). WB, ICC/IF; Rat.Read more (PubMed: 21138835) »
- Molenaar Ret al. Expression of retinaldehyde dehydrogenase enzymes in mucosal dendritic cells and gut-draining lymph node stromal cells is controlled by dietary vitamin A. J Immunol 186:1934-42 (2011).Read more (PubMed: 21220692) »
See all 5 publications for this product
Publishing research using ab23375? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"