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ab25700 |
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Read our guarantee »Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Anti-ALDH1A1 antibody - Neuronal Marker
See all ALDH1A1 products (13) ...
Rabbit polyclonal to ALDH1A1 - Neuronal Marker
Rabbit polyclonal to ALDH1A1 (ab23375) detects ALDH1A1 at the predicted MW (54 kDa). The immunogen used to generate ab23375 will also detect ALDH1A7 [P13601] with 100% identity.
IHC-Fr, ICC/IF, IHC-FrFl, IHC-P, WBmore details
Reacts with
Mouse, Rat, Sheep, Goat, Dog, Human
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Rat ALDH1A1.
(Peptide available as ab25700.)
This antibody gave a positive result in the following lysates: Mouse Brain Tissue, Mouse Liver Tissue, Rat Liver Tissue, Mouse Embryonic Brain (E14) Tissue and HepG2.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Cancer >> Tumor biomarkers >> Other
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Our Abpromise guarantee covers the use of ab23375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-Fr: 1/200 - 1/400 (See Abreview).
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
Cofactor metabolism; retinol metabolism.
Belongs to the aldehyde dehydrogenase family.
Cytoplasm.
Target information above from: UniProt accessionP00352
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Immunocytochemistry/ Immunofluorescence - ALDH1A1 antibody - Neuronal Marker (ab23375)

ICC/IF image of ab23375 stained rat PC12 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab23375, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ALDH1A1 antibody - Neuronal Marker (ab23375)

IHC image of ALDH1A1 staining in rat brain FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23375, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
- ALDH1A1 antibody - Neuronal Marker (ab23375)

ab23375 staining ALDH1A1 in Mouse hypothalamic tanycytes by IHC-FrFl (Immunohistochemistry Free-floating). 40 µm free-floating sections were incubated with primary antibody (1/300) for 18 hours at 4°C in blocking solution. An Alexa Fluor® 555-conjugated Donkey polyclonal to rabbit IgG (1/300) was used as secondary antibody.
This image is courtesy of an anonymous Abreview
Western blot - ALDH1A1 antibody - Neuronal Marker (ab23375)

All lanes : Anti-ALDH1A1 antibody - Neuronal Marker (ab23375) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 54 kDa
Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 90 seconds
Western blot - ALDH1A1 antibody - Neuronal Marker (ab23375)

All lanes : Anti-ALDH1A1 antibody - Neuronal Marker (ab23375) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lane 4 : E14 Mouse Embryo Brain Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 51,54 kDa (why is the actual band size different from the predicted?)
Exposure time : 90 seconds
This product has been referenced in:
See all 5 publications for this product
Publishing research using ab23375? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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ICC/IF image of ab23375 stained rat PC12 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab23375, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

IHC image of ALDH1A1 staining in rat brain FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23375, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

ab23375 staining ALDH1A1 in Mouse hypothalamic tanycytes by IHC-FrFl (Immunohistochemistry Free-floating). 40 µm free-floating sections were incubated with primary antibody (1/300) for 18 hours at 4°C in blocking solution. An Alexa Fluor® 555-conjugated Donkey polyclonal to rabbit IgG (1/300) was used as secondary antibody.
This image is courtesy of an anonymous Abreview

All lanes : Anti-ALDH1A1 antibody - Neuronal Marker (ab23375) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 54 kDa
Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 90 seconds

All lanes : Anti-ALDH1A1 antibody - Neuronal Marker (ab23375) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lane 4 : E14 Mouse Embryo Brain Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 51,54 kDa (why is the actual band size different from the predicted?)
Exposure time : 90 seconds






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