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Western blot - ALDH1A1 antibody - Neuronal Marker (ab23375)
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Immunocytochemistry/ Immunofluorescence - ALDH1A1 antibody - Neuronal Marker (ab23375)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ALDH1A1 antibody - Neuronal Marker (ab23375)
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Product Name
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ALDH1A1 antibody - Neuronal Marker
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See all ALDH1A1 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ALDH1A1 - Neuronal Marker
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Rat ALDH1A1.
(Peptide available as ab25700.)
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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Rabbit polyclonal to ALDH1A1 (ab23375) detects ALDH1A1 at the predicted MW (54 kDa). The immunogen used to generate ab23375 will also detect ALDH1A7 [P13601] with 100% identity.
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-FrFl, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-Fr: 1/200 - 1/400 (See Abreview).
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive result in the following lysates:
Mouse Brain Tissue, Mouse Liver Tissue, Mouse Kidney Tissue, Mouse Testis Tissue, Rat Liver Tissue, Rat Heart Tissue, Rat Kidney Tissue, HepG2 Whole Cell
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Cellular localization
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Cytoplasmic
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
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Relevance
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ALDH1A1 is an aldehyde dehydrogenase able to oxidize a wide variety of aliphatic aldehydes (retinaldehyde, acetaldehyde, etc.) to the corresponding carboxylic acids (retinoic acid, acetic acid, etc.). ALDH1A1 (also known as RALDH1, ALDH1, or AHD2) is highly expressed in the dorsal retina, ventral midbrain (dopaminergic neurons), and hematopoietic stem cells. ALDH1A1 is involved in retinoic acid (RA) synthesis during vertebrate embryogenesis. ALDH1A1 is first detected at E9.0-E10.5 in cranial tissues (ventral mesencephalon, dorsal retina, thymic primordia, optic vesicles) and in the mesonephros. ALDH1A1 is also of interest in Parkinson's Disease (PD) being expressed in the the A9 dopaminergic (DA) neuronal group projecting to the dorsal striatum; this is the most vulnerable site in PD (Chung et al, 2005).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.30 - 0.30 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ALDH1A1 antibody - Neuronal Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ALDH1A1 antibody - Neuronal Marker images, references, products related to ab23375 and other tools.
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ALDH1A1 antibody - Neuronal Marker images:
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 Western blot - ALDH1A1 antibody - Neuronal Marker (ab23375)
All lanes : ALDH1A1 antibody - Neuronal Marker (ab23375) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 3 : Kidney (Mouse) Tissue Lysate
Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 5 : Liver (Rat) Tissue Lysate
Lane 6 : Heart (Rat) Tissue Lysate
Lane 7 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 54 kDa
Additional bands at : 100 kDa,23 kDa,40 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - ALDH1A1 antibody - Neuronal Marker (ab23375)
ICC/IF image of ab23375 stained rat PC12 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab23375, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ALDH1A1 antibody - Neuronal Marker (ab23375)
IHC image of ALDH1A1 staining in rat brain FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23375, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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- ALDH1A1 antibody - Neuronal Marker (ab23375)
ab23375 staining ALDH1A1 in Mouse hypothalamic tanycytes by IHC-FrFl (Immunohistochemistry Free-floating). 40 µm free-floating sections were incubated with primary antibody (1/300) for 18 hours at 4°C in blocking solution. An Alexa Fluor® 555-conjugated Donkey polyclonal to rabbit IgG (1/300) was used as secondary antibody. This image is courtesy of an anonymous Abreview
See Abreview
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Western blot - ALDH1A1 antibody - Neuronal Marker (ab23375)
All lanes : ALDH1A1 antibody - Neuronal Marker (ab23375) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 54 kDa
Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 90 seconds
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References for ALDH1A1 antibody - Neuronal Marker (ab23375)
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This product has been used in: (two most recent references)
Shearer KD et al. Photoperiodic regulation of retinoic acid signaling in the hypothalamus. J Neurochem 112:246-57 (2010). IHC-FoFr; Mouse. PubMed: 19860856
van de Pavert SA et al. Chemokine CXCL13 is essential for lymph node initiation and is induced by retinoic acid and neuronal stimulation. Nat Immunol 10:1193-9 (2009). IHC-Fr; Mouse. PubMed: 19783990
If you publish research using ab23375 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ALDH1A1
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