Anti-ALDH1A1 antibody (ab227964)
Key features and details
- Rabbit polyclonal to ALDH1A1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ALDH1A1 antibody
See all ALDH1A1 primary antibodies -
Description
Rabbit polyclonal to ALDH1A1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Rabbit, Cow, Dog, Chimpanzee -
Immunogen
Recombinant fragment within Human ALDH1A1 (internal sequence). The exact sequence is proprietary.
Database link: P00352 -
Positive control
- WB: A549 whole cell lysate; Mouse and rat liver lysates. IHC-P: Human gastric cancer tissue. ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/5000 - 1/20000. Predicted molecular weight: 55 kDa.
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ICC/IF |
1/100 - 1/1000.
Methanol fixed. |
Notes |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/5000 - 1/20000. Predicted molecular weight: 55 kDa. |
ICC/IF
1/100 - 1/1000. Methanol fixed. |
Target
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Function
Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. -
Pathway
Cofactor metabolism; retinol metabolism. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 464529 Chimpanzee
- Entrez Gene: 281615 Cow
- Entrez Gene: 216 Human
- Entrez Gene: 11668 Mouse
- Entrez Gene: 24188 Rat
- Entrez Gene: 443343 Sheep
- Omim: 100640 Human
- SwissProt: P48644 Cow
see all -
Alternative names
- Acetaldehyde dehydrogenase 1 antibody
- AHD2 antibody
- AL1A1_HUMAN antibody
see all
Images
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Paraffin-embedded human gastric cancer tissue stained for ALDH1A1 using ab227964 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: EDTA based, pH 8.0 buffer, 15 mins.
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Anti-ALDH1A1 antibody (ab227964) at 1/10000 dilution + A549 (human lung carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa7.5% SDS-PAGE gel.
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Methanol-fixed A549 (human lung carcinoma cell line) cells stained for ALDH1A1 (green) using ab227964 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
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Anti-ALDH1A1 antibody (ab227964) at 1/5000 dilution + Mouse liver lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa10% SDS-PAGE gel.
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Anti-ALDH1A1 antibody (ab227964) at 1/5000 dilution + Rat liver lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa10% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab227964 has been referenced in 5 publications.
- Wang T et al. A SNP of miR-146a is involved in bladder cancer relapse by affecting the function of bladder cancer stem cells via the miR-146a signallings. J Cell Mol Med 24:8545-8556 (2020). PubMed: 32596945
- Castagnoli L et al. WNT signaling modulates PD-L1 expression in the stem cell compartment of triple-negative breast cancer. Oncogene 38:4047-4060 (2019). Human . PubMed: 30705400
- Fletcher PA et al. Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells. Front Endocrinol (Lausanne) 10:623 (2019). ICC/IF ; Rat . PubMed: 31620083
- Chen L et al. TRAF6 regulates tumour metastasis through EMT and CSC phenotypes in head and neck squamous cell carcinoma. J Cell Mol Med 22:1337-1349 (2018). WB ; Human . PubMed: 29193723
- Huang CF et al. NLRP3 inflammasome activation promotes inflammation-induced carcinogenesis in head and neck squamous cell carcinoma. J Exp Clin Cancer Res 36:116 (2017). WB, IHC-P, ICC/IF ; Mouse . PubMed: 28865486