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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab9883? Please let us know so that we can cite the reference in this datasheet
ab9883 has been referenced in 4 publications.
- Dylla SJ et al. Colorectal cancer stem cells are enriched in xenogeneic tumors following chemotherapy. PLoS ONE 3:e2428 (2008). IHC-Fr; Human. PubMed: 18560594
- Yoon M et al. Developmental expression of aldehyde dehydrogenase in rat: a comparison of liver and lung development. Toxicol Sci 89:386-98 (2006). PubMed: 16291827
- Szatmari I et al. PPARgamma controls CD1d expression by turning on retinoic acid synthesis in developing human dendritic cells. J Exp Med 203:2351-62 (2006). PubMed: 16982809
- Iwata M et al. Retinoic acid imprints gut-homing specificity on T cells. Immunity 21:527-38 (2004). PubMed: 15485630
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - ALDH1A1 antibody (ab9883)
Anti-ALDH1A1 antibody (ab9883) at 0.03 µg/ml + Human Liver lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size : 55 kDa
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (ab9883)
IHC image of ab9883 staining in Human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9883, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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