Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab9883 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
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We’ll take the goat polyclonal ab115252. Can you send us the protocol that you recommend for this antibody? |
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ANSWER: |
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab115252. This product is currently out of stock and delivery is expected around November 9. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research. |
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It would be best for us is we can get a replacement for ab9883. Thank you, |
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ANSWER: |
Thank you for your reply. As we have already sent a vial of ab9883 as a replacement and neither ab9883 vial has produced acceptable results, we generally would send a different antibody to try. We also have a mouse monoclonal ab105920, and a different goat polyclonal ab115252 that have been tested in human IHC-P: http://www.abcam.com/ALDH1A1-antibody-5A11-ab105920.html http://www.abcam.com/ALDH1A1-antibody-ab115252.html Please let me know if one of these is acceptable and I will send it to you promptly. |
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I’m having trouble finding the PO number. I know the original order was placed around 2/16/11. We changed which staff was taking care of orders at the end of February so I’m not sure if I can find the PO number. We must use the antibody whose host is not rabbit since later we will try to do double staining and the other antibody we use is rabbit antibody. Therefore we can not use the ab23375. Thanks |
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ANSWER: |
Thank you for your reply. I think we have found the original order from February, but as we have sent a replacement vial of ab9883 which also did not work, in this case it might be best to issue a credit note or refund since the rabbit polyclonal ab23375 can not be used. Please let me know if this is an acceptable solution and I will issue the credit/refund immediately. Let me know if you have any questions or if there is anything else we can do for you. |
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Can you send us the same one that we were trying to use (ab9883)? |
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ANSWER: |
Thank you for your reply. Could you please provide your original order or PO number? I did find an order that went to your institution, however we had already sent a replacement vial of ab9883 on that order. If you have not been able to obtain satisfactory results with two vials of ab9883, an alternative antibody may work better in your application. As Caitlin had mentioned, ab23375 is highly rated and would also be guaranteed for your experiment. Alternatively, I would be happy to offer you a credit or refund. Please let me know your original order or PO number and how you would like to proceed and I am happy to assist you further. |
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What is the host animal of the other antibody? Does it work with paraffin embedded sections? What is the cross reactivity? We’ll decide which one we want based on these questions. Thank you |
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ANSWER: |
Thanks for your reply.
We have a few other ALDH1A1 antibodies but this is our most popular and has very good reviews/publications. It is a rabbit polyclonal, it does work in paraffin sections, and it has been tested for cross-reactivity with human, mouse, rat, dog, goat, and sheep samples.
Please let me know if you have any other questions. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-ALDH1A1 antibody (ab9883) at 0.03 µg/ml + Human Liver lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size : 55 kDa
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
IHC image of ab9883 staining in Human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9883, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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