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Overview

  • Product nameALDH1A1 protein (Tagged-His Tag)See all ALDH1A1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 255-501 (247 amino acids) of Human ALDH1A1 with an N terminal His tag. observed mol wt: 29 kDa; UniProt ID: P00352.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 135 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesKEAAGKSNLKRVTLELGGKSPCIVLADADLDNAVEFAHHG VFYHQGQCCIAASR IFVEESIYDEFVRRSVERAKKYIL GNPLTPGVTQGPQIDKEQYDKILDLIESGK KEGAKLEC GGGPWGNKGYFVQPTVFSNVTDEMRIAKEEIFGPVQQIMK FKSLDD VIKRANNTFYGLSAGVFTKDIDKAITISSALQ AGTVWVNCYGVVSAQCPFGGFK MSGNGRELGEYGFHEY TEVKTVTVKISQKNS
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91895 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Acetaldehyde dehydrogenase 1AHD2AL1A1_HUMAN
        ALDCAldehyde DehydrogenaseAldehyde dehydrogenase 1 family member A1Aldehyde dehydrogenase 1 solubleAldehyde dehydrogenase 1A1Aldehyde dehydrogenase cytosolicAldehyde dehydrogenase family 1 member A1Aldehyde dehydrogenase liver cytosolicALDH 1ALDH 1A1ALDH class 1ALDH-E1ALDH1ALDH1 A1ALDH11ALDH1A1ALHDIIcytosolicMGC2318PUMB1RALDH 1RalDH1Retinal dehydrogenase 1
      see all
  • FunctionBinds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
  • PathwayCofactor metabolism; retinol metabolism.
  • Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession P00352 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    ALDH1A1 protein (Tagged-His Tag) images

    • Western blot - ALDH1A1 protein (Tagged-His Tag) (ab91895)
      Western blot - ALDH1A1 protein (Tagged-His Tag) (ab91895)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for ALDH1A1 protein (Tagged-His Tag) (ab91895)

    ab91895 has not yet been referenced specifically in any publications.

    Publishing research using ab91895 ? Please let us know so that we can cite the reference in this datasheet

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