![Immunocytochemistry/ Immunofluorescence - Anti-ALDH2 antibody [9A10AF10 ] (ab110311) image](#ALDH2-Primary-antibodies-ab110311-3.gif)
![Immunoprecipitation - Anti-ALDH2 antibody [9A10AF10 ] (ab110311) image](#ALDH2-Primary-antibodies-ab110311-4.gif)
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Product name
Anti-ALDH2 antibody [9A10AF10 ]
See all ALDH2 products (9) ...
Description
Mouse monoclonal [9A10AF10 ] to ALDH2
Tested applications
ICC/IF, IP, In-Cell ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Positive control
HepG2 cells; Human, Rat and Mouse liver and HepG2 cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
ab110311 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
9A10AF10
Isotype
IgG2a
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Heart disease
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Alcohol metabolism
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cardiovascular >> Heart >> Cardiac metabolism
Cardiovascular >> Atherosclerosis >> Ischemia / Reperfusion
Signal Transduction >> Metabolism >> Alcohol metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml. For 2 hours.
IP: Use at an assay dependent dilution.
In-Cell ELISA: Use a concentration of 5 µg/ml.
Target
Pathway
Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
Sequence similarities
Belongs to the aldehyde dehydrogenase family.
Cellular localization
Mitochondrion matrix.
Target information above from: UniProt accessionP05091
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Acetaldehyde dehydrogenase 2 antibody
- Aldehyde dehydrogenase 2 family (mitochondrial) antibody
- Aldehyde dehydrogenase 2 family antibody
see all
Database links
- Entrez Gene: 508629 Cow
- Entrez Gene: 217 Human
- Entrez Gene: 11669 Mouse
- Entrez Gene: 29539 Rat
- Omim: 100650 Human
- SwissProt: P20000 Cow
- SwissProt: P05091 Human
- SwissProt: P47738 Mouse
see all
Anti-ALDH2 antibody [9A10AF10 ] images:
Immunocytochemistry/ Immunofluorescence - Anti-ALDH2 antibody [9A10AF10 ] (ab110311)
![Immunocytochemistry/ Immunofluorescence - Anti-ALDH2 antibody [9A10AF10 ] (ab110311)](/ps/datasheet/images/110/ab110311/ALDH2-Primary-antibodies-ab110311-3.gif)
Immunocytochemistry analysis using ab110311 at 5µg/ml staining ALDH2 in HepG2 cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
Note: Target protein locates mainly in the mitochondrial matrix.
Immunoprecipitation - Anti-ALDH2 antibody [9A10AF10 ] (ab110311)
![Immunoprecipitation - Anti-ALDH2 antibody [9A10AF10 ] (ab110311)](/ps/datasheet/images/110/ab110311/ALDH2-Primary-antibodies-ab110311-4.gif)
Using MitoSciences' standard immunoprecipitation protocol, ab110311 precipitates down the 54kDa mature ALDH2 protein from human, mouse, and rat liver tissue lysate and HepG2 culture cell lysate. Identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.
References for Anti-ALDH2 antibody [9A10AF10 ] (ab110311)
ab110311 has not yet been referenced specifically in any publications.
Publishing research using ab110311? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"