Anti-ALDH3A1 antibody (ab76976)

Overview

• Product nameAnti-ALDH3A1 antibodySee all ALDH3A1 primary antibodies ...
• Description
  Rabbit polyclonal to ALDH3A1
• SpecificityThis antibody is predicted to have no cross reactivity to ALDH3A2.
• Tested applicationsWB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide (Human): a 13 amino acid peptide from near the C terminus of human ALDH3A1. (NP_000682).

• Positive controlHuman stomach tissue lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Alcohol metabolism

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Signal Transduction
  • Metabolism
  • Alcohol metabolism

  • Neuroscience
  • Sensory System
  • Visual system

  • Cell Biology
  • Other Antibodies
  • Oxidative Stress

Applications

Target

• FunctionALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes.
• Tissue specificityHigh levels in stomach, esophagus and lung; low level in the liver and kidney.
• Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession P30838 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 218 Human
  • Entrez Gene: 11670 Mouse
  • Entrez Gene: 25375 Rat
  • Omim: 100660 Human
  • SwissProt: P30838 Human
  • SwissProt: P47739 Mouse
  • SwissProt: P11883 Rat
  • Unigene: 531682 Human

  • Unigene: 4257 Mouse
  • Unigene: 105627 Rat

  see all

• Alternative names
  AHD 4 antibodyAHD C antibodyAhd4 antibody

  AHDC antibodyAL3A1_HUMAN antibodyAldehyde dehydrogenase 3 antibodyAldehyde dehydrogenase 3 family member A1 antibodyAldehyde dehydrogenase 3 family, member A1 antibodyAldehyde dehydrogenase 3A1 antibodyAldehyde dehydrogenase antibodyAldehyde dehydrogenase class 3 antibodyAldehyde dehydrogenase dimeric NADP preferring antibodyAldehyde dehydrogenase family 3 member A1 antibodyAldehyde dehydrogenase family 3 subfamily A1 antibodyAldehyde dehydrogenase isozyme 3 antibodyAldehyde dehydrogenase type III antibodyAldehyde dehydrogenase, dimeric NADP preferring antibodyALDH 3 antibodyALDH 3A1 antibodyALDH III antibodyALDH3 A1 antibodyALDH3 antibodyALDH3A 1 antibodyAldh3a1 antibodyALDHIII antibodydimeric NADP-preferring antibodyMGC10406 antibodyStomach aldehyde dehydrogenase antibodyTumor associated aldehyde dehydrogenase tumor ALDH antibody

  see all

Anti-ALDH3A1 antibody images

• 

  Western blot - ALDH3A1 antibody (ab76976)
  Lane 1 : Anti-ALDH3A1 antibody (ab76976) at 1 µg/ml
  Lane 2 : Anti-ALDH3A1 antibody (ab76976) at 2 µg/ml
  
  Lane 1 : human stomach lysate
  Lane 2 : human stomach lysate
  
  Lysates/proteins at 15 µg per lane.
  
  
  Predicted band size : 50 kDa
  Observed band size : 50 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody (ab76976)

  IHC image of ALDH3A1 staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76976, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.