Overview

  • Product name
  • Description
    Goat polyclonal to ALDH5A1
  • Specificity
    This antibody is expected to recognize both reported isoforms (NP_733936.1; NP_001071.1)
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide:

    SQDPAQIWRVAE

    by a Cysteine residue linker, corresponding to internal sequence amino acids 472-483 of Human ALDH5A1 (NP_733936.1; NP_001071.1)

  • Positive control
    • Human, Mouse and Rat Liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab106823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 57 kDa.
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA).
  • Tissue specificity
    Brain, pancreas, heart, liver, skeletal muscle and kidney. Lower in placenta.
  • Pathway
    Amino-acid degradation; 4-aminobutanoate degradation.
  • Involvement in disease
    Defects in ALDH5A1 are the cause of succinate semialdehyde dehydrogenase deficiency (SSADH deficiency) [MIM:271980]. SSADH deficiency is a rare inborn error in the metabolism of 4-aminobutyric acid (GABA) which leads to accumulation of 4-hydroxybutyric acid in physiologic fluids of patients. The disease is characterized by severe ataxia and by mildly retarded psychomotor development.
  • Sequence similarities
    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aldedehyde dehydrogenase 5 family antibody
    • Aldehyde dehydrogenase 5 family member A1 antibody
    • Aldehyde dehydrogenase 5A1 antibody
    • Aldehyde dehydrogenase family 5 member A1 antibody
    • ALDH5A 1 antibody
    • Aldh5a1 antibody
    • mitochondrial antibody
    • Mitochondrial succinate semialdehyde dehydrogenase antibody
    • NAD(+) dependent succinic semialdehyde dehydrogenase antibody
    • NAD(+)-dependent succinic semialdehyde dehydrogenase antibody
    • SSADH antibody
    • SSDH antibody
    • SSDH_HUMAN antibody
    • Succinate semialdehyde dehydrogenase antibody
    • Succinate-semialdehyde dehydrogenase antibody
    see all

Images

  • ab106823 (5µg/ml) shows staining of ALDH5A1 in paraffin embedded Human Liver following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows heavy textured cystoplasm staining in hepatocytes, consistent with mitochondia.

  • Anti-ALDH5A1 antibody (ab106823) at 0.5 µg/ml + Human Liver lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 57 kDa

References

ab106823 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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