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Anti-ALK antibody [5A4] (ab17127)

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Overview

Product name

Anti-ALK antibody [5A4]
See all ALK products (14) ...

Description

Mouse monoclonal [5A4] to ALK

Tested applications

IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Prokaryotic recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of NPM-ALK transcript (419-520 aa).

Positive control

Anaplastic lymphoma.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, Tissue culture supernatant

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

5A4

Isotype

IgG1

Research areas

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases

Applications

Show applications key

Our Abpromise guarantee covers the use of ab17127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    IHC-P

     IHC-P: 1/25 - 1/70.Perfo...Read more →

    IHC-P: 1/25 - 1/70.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

Function

Orphan receptor with a tyrosine-protein kinase activity. Appears to play an important role in the normal development and function of the nervous system. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif.

Tissue specificity

Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.

Involvement in disease

Note=A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas.
Note=A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A.
Note=A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.
Defects in ALK are the cause of susceptibility to neuroblastoma type 3 (NBLST3) [MIM:613014]. Neuroblastoma is a common neoplasm of early childhood arising from embryonic cells that form the primitive neural crest and give rise to the adrenal medulla and the sympathetic nervous system.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 1 LDL-receptor class A domain.
Contains 2 MAM domains.
Contains 1 protein kinase domain.

Post-translational
modifications

N-glycosylated.

Cellular localization

Membrane.

Target information above from: UniProt accessionQ9UM73 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Alk antibody
  • ALK tyrosine kinase receptor antibody
  • ALK Tyrosine Kinase Receptor Precursor antibody
  • ALK/EML4 fusion gene, included antibody
  • ALK/NPM1 fusion gene, included antibody
  • ALK_HUMAN antibody
  • anaplastic lymphoma kinase (Ki-1) antibody
  • Anaplastic lymphoma kinase antibody
  • Anaplastic lymphoma kinase Ki1 antibody
  • Anaplastic Lymphoma Kinase p80 antibody
  • Anaplastic Lymphoma Kinase p80 antibody
  • CD246 antibody
  • CD246 Antigen antibody
  • EC 2.7.10.1 antibody
  • Ki 1 antibody
  • Ki1 antibody
  • NBLST3 antibody
  • Tcrz antibody
  • TFG/ALK antibody
see all

Database links

References for Anti-ALK antibody [5A4] (ab17127)

This product has been referenced in:

  • Kijima Tet al. Favorable response to crizotinib in three patients with echinoderm microtubule-associated protein-like 4-anaplastic lymphoma kinase fusion-type oncogene-positive non-small cell lung cancer. Cancer Sci 102:1602-4 (2011). IHC; Human.Read more (PubMed: 21767331) »
  • Sasaki Tet al. A Novel ALK Secondary Mutation and EGFR Signaling Cause Resistance to ALK Kinase Inhibitors. Cancer Res 71:6051-60 (2011). IHC-P; Human.Read more (PubMed: 21791641) »

See all 3 publications for this product

Publishing research using ab17127? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"