|
|
|
|
|
Product Name
|
ALK antibody [5A4]
|
| |
See all ALK antibodies (12)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [5A4] to ALK
|
|
Immunogen
|
Prokaryotic recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of NPM-ALK transcript (419-520 aa).
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
IHC-P
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IHC-P: 1/25 - 1/70. An incubation period of 60 minutes at room temperature is recommendedc using an ABC method. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Anaplastic lymphoma.
|
|
Cellular localization
|
Type I membrane protein
|
|
Research areas
|
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
|
|
Relevance
|
Anaplastic lymphoma kinase (ALK) was originally discovered as a NPM (Nucleophosmin)-ALK fusion protein. ALK and LTK (leukocyte tyrosine kinase) are receptor tyrosine kinases similar to the insulin receptor subfamily of kinases. LTK is found in murine B Lymphocyte precursors and in forebrain neurons. ALK is usually found in the nervous system. A truncated form of ALK occurs in many non Hodgkin's lymphomas.
The ALK gene is on chromosome 2. Upon translocation between chromosome 2 and chromosome 5 [t(2;5}], the ALK gene fuses with the NPM gene. NPM, also known as B23, is an ubiquitous nucleolar phosphoprotein involved in the transport of pre ribosomal components between the nucleolus and the cytoplasm. The chimeric product (NPM ALK) resulting from t(2;5) translocation is a protein of 80kDa with the N terminal portion of NPM linked to the complete intracellular portion of ALK. Karpas 299 and SUDHL1 lymphoid cell lines strongly express NPM ALK.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
5A4
|
|
Isotype
|
IgG1
|
|
Purity
|
Tissue culture supernatant
|
|
Storage buffer
|
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, Tissue culture supernatant Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
Concentration not determined - why is this?
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ALK antibody [5A4] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
|
Search PubMed (MEDLINE) for references to ALK
|
| |
ALK antibody [5A4] - more information
|
Customer reviews (feedback) regarding ALK antibody [5A4] |
Customer FAQs regarding ALK antibody [5A4] |
Protocols for ALK antibody [5A4] |
Price and availability of products related to ALK antibody [5A4] |
| |
ALK antibody [5A4] - related products:
|
|
|
| |
ALK antibody [5A4] - other tools:
|
Contact Abcam with a Technical Enquiry about ALK antibody [5A4] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
