![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ALK antibody [SP8] (ab16670) image](#abcam1-16670.jpg)
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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Overview
Product name
Anti-ALK antibody [SP8]
See all ALK products (14) ...
Description
Rabbit monoclonal [SP8] to ALK
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: WQHQP EDRP NFAIIL ERI EYCTQDP DV INTALPIE Y GPLVEEEEK VPVRPKDPEG VPPLLVSQQ A KREEERSP AA PPPLPTT SSG KAAKKP TAAE VSVRV PRG, corresponding to amino acids 1366-1468 of Human ALK, a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript.
WQHQP EDRPNFAIIL ERIEYCTQDP DVINTALPIE Y GPLVEEEEK VPVRPKDPEG VPPLLVSQQA KREEERSP AA PPPLPTTSSG KAAKKPTAAE VSVRVPRG
Positive control
Anaplastic lymphoma
General notes
The amino acid sequence used to raise this antibody was: aa 426-528.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Purity
Tissue culture supernatant
Primary antibody notes
This antibody recognises a human p80 protein, identified as a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in a third of large cell lymphomas. This rabbit polyclonal antibody can be used to detect p80 in these lymphomas and may also be used to detect a recently described subtype of large B cell lymphoma, which expresses the full-length ALK protein.
Clonality
Monoclonal
Clone number
SP8
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ALK antibody [SP8] (ab16670)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ALK antibody [SP8] (ab16670) image](/ps/datasheet/Images/16/ab16670/abcam1-16670.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab16670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
Application notes
IHC-P: 1/100. Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Orphan receptor with a tyrosine-protein kinase activity. Appears to play an important role in the normal development and function of the nervous system. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif.
Tissue specificity
Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.
Involvement in disease
Note=A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas.
Note=A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A.
Note=A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.
Defects in ALK are the cause of susceptibility to neuroblastoma type 3 (NBLST3) [MIM:613014]. Neuroblastoma is a common neoplasm of early childhood arising from embryonic cells that form the primitive neural crest and give rise to the adrenal medulla and the sympathetic nervous system.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 1 LDL-receptor class A domain.
Contains 2 MAM domains.
Contains 1 protein kinase domain.
Post-translational
modifications
N-glycosylated.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ9UM73
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alk antibody
- ALK tyrosine kinase receptor antibody
- ALK Tyrosine Kinase Receptor Precursor antibody
see all
Database links
- Entrez Gene: 238 Human
- Omim: 105590 Human
- SwissProt: Q9UM73 Human
- Unigene: 654469 Human
Anti-ALK antibody [SP8] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ALK antibody [SP8] (ab16670)
Human anaplastic lymphoma stained with ab16670.
References for Anti-ALK antibody [SP8] (ab16670)
This product has been referenced in:
- Lagarrigue Fet al. Matrix metalloproteinase-9 is upregulated in nucleophosmin-anaplastic lymphoma kinase-positive anaplastic lymphomas and activated at the cell surface by the chaperone heat shock protein 90 to promote cell invasion. Cancer Res 70:6978-87 (2010). IHC-P; Human.Read more (PubMed: 20699364) »
- Takeuchi Ket al. KIF5B-ALK, a novel fusion oncokinase identified by an immunohistochemistry-based diagnostic system for ALK-positive lung cancer. Clin Cancer Res 15:3143-9 (2009). IHC-P; Human.Read more (PubMed: 19383809) »
See all 2 publications for this product
Publishing research using ab16670? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"