ALK antibody [SP8] (ab16670)

Product Name 

ALK antibody [SP8]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [SP8] to ALK

Immunogen 

Recombinant fragment: SEGTS PGSKMALQSS FTCWNGTVLQ LGQACDFHQD CAQGEDESQM CRKLPVGFYCNFEDGFCGWT QGTLSPHTPQ WQVRTLKDAR FQDHQDHALL LSTTDVPA, corresponding to amino acids 426-528 of Human ALK, a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript.
BLAST 'SEGTS PGSK MALQSS FTC WNGTVLQ LG QACDFHQD C AQGEDESQM CRKLPVGFYC NFEDGFCGWT QGTLSPHTP Q WQVRTLKD AR FQDHQDH ALL LSTTDV ' with or

Reacts with 
(species key)

Hu

Tested applications 
(see key)

IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/100. Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Anaplastic lymphoma

Cellular localization 

Type I membrane protein

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases

Relevance 

Anaplastic lymphoma kinase (ALK) was originally discovered as a NPM (Nucleophosmin)-ALK fusion protein. ALK and LTK (leukocyte tyrosine kinase) are receptor tyrosine kinases similar to the insulin receptor subfamily of kinases. LTK is found in murine B Lymphocyte precursors and in forebrain neurons. ALK is usually found in the nervous system. A truncated form of ALK occurs in many non Hodgkin's lymphomas.
The ALK gene is on chromosome 2. Upon translocation between chromosome 2 and chromosome 5 [t(2;5}], the ALK gene fuses with the NPM gene. NPM, also known as B23, is an ubiquitous nucleolar phosphoprotein involved in the transport of pre ribosomal components between the nucleolus and the cytoplasm. The chimeric product (NPM ALK) resulting from t(2;5) translocation is a protein of 80kDa with the N terminal portion of NPM linked to the complete intracellular portion of ALK. Karpas 299 and SUDHL1 lymphoid cell lines strongly express NPM ALK.

This antibody recognises a human p80 protein, identified as a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in a third of large cell lymphomas.
This rabbit polyclonal antibody can be used to detect p80 in these lymphomas and may also be used to detect a recently described subtype of large B cell lymphoma, which expresses the full-length ALK protein.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

SP8

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.1% Sodium Azide

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C.

Notes 

The amino acid sequence used to raise this antibody was: aa 426-528.

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ALK antibody [SP8] images:

References for ALK antibody [SP8] (ab16670)

  This product has been used in:

Takeuchi K et al. KIF5B-ALK, a novel fusion oncokinase identified by an immunohistochemistry-based diagnostic system for ALK-positive lung cancer. Clin Cancer Res 15:3143-9 (2009). IHC-P; Human. PubMed: 19383809

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