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I wish to use this in an immunohistochemical application (no parafin, just paraformaldehyde-fixed, frozen sections). You report that this antiserum works for IHC. Do you have any photos, or information on dilution conditions? |
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ANSWER: |
Thank you for your enquiry. I have contacted the source of the antibody to determine further details of the immunohistochemistry. They have clarified that this antibody can be applied using both frozen sections and paraffin embedded sections. Unfortunately they could not provide me with details of a recommended dilution or protocol. I would advise you trying a range of dilutions initially and modifying according to the results obtained. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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I have purchased the rabbit polyclonal ALK antibody and have had success with it in IHC but not by Western blot and was wondering if you sell the blocking peptide for the ALK antibody. |
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ANSWER: |
Thank you for your enquiry. We would like to inform you that this antibody has not been tested for Western blot application so we do not know if it works in this assay. We regret to inform you that we do not sell any blocking peptide for the ALK antibody.
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Hi Neil, it was brought to my attention that you have a new product, namely ALK antibody (ab4061)Rabbit polyclonal to ALK. I would greatly appreciate it if you could send us a test sample of the antibody to try out on our sections as we have been using another antibody for ALK produced by Santa Cruz Biotech and we are not altogether happy with it. Regards Mark Carmody _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Mark Carmody PhD Developmental Genetics Lab. Room 3.33 Dept. of Biochemistry Biosciences Institute University College Cork Cork Ireland. Phone: +353 21 4901361 Fax: +353 21 4274034 |
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ANSWER: |
Thanks for your enquiry. This antibody has already been tested in IHC and so we would not give out any samples for an application that we know this antibody already works in. We have a money-back guarantee for all our abs in the applications that are listed on the datasheet. Good luck with your experiments. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Ab4061 staining Human normal colon. Staining is localized to nucleus and membrane.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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