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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ALK antibody(ab4061)
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Product Name
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ALK antibody
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See all ALK antibodies (12)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ALK
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Immunogen
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Recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the c-terminus of the NPM-Alk transcript. The exact sequence is considered commercially sensitive.
BLAST 'SEGTS PGSK MALQSS FTC WNGTVLQ LG QACDFHQD C AQGEDESQM CRKLPVGFYC NFEDGFCGWT QGTLSPHTP Q WQVRTLKD AR FQDHQDH ALL LSTTDV ' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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Recognizes a human p80 protein, identified as a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in a third of large cell lymphomas. This rebbit polyclonal antibody can be used to detect p80 in these lymphomas and may also be used to detect a recently described subtype of large B cell lymphoma, which expresses the full-length ALK protein.
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Tested applications
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IHC-Fr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 2 µg/ml.
IHC-Fr: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Anaplastic lymphoma.
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Cellular localization
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Type I membrane protein
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Research areas
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Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
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Relevance
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Anaplastic lymphoma kinase (ALK) was originally discovered as a NPM (Nucleophosmin)-ALK fusion protein. ALK and LTK (leukocyte tyrosine kinase) are receptor tyrosine kinases similar to the insulin receptor subfamily of kinases. LTK is found in murine B Lymphocyte precursors and in forebrain neurons. ALK is usually found in the nervous system. A truncated form of ALK occurs in many non Hodgkin's lymphomas.
The ALK gene is on chromosome 2. Upon translocation between chromosome 2 and chromosome 5 [t(2;5}], the ALK gene fuses with the NPM gene. NPM, also known as B23, is an ubiquitous nucleolar phosphoprotein involved in the transport of pre ribosomal components between the nucleolus and the cytoplasm. The chimeric product (NPM ALK) resulting from t(2;5) translocation is a protein of 80kDa with the N terminal portion of NPM linked to the complete intracellular portion of ALK. Karpas 299 and SUDHL1 lymphoid cell lines strongly express NPM ALK.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ALK antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ALK antibody images, references, products related to ab4061 and other tools.
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ALK antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ALK antibody(ab4061)
Ab4061 staining Human normal colon. Staining is localized to nucleus and membrane.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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Search PubMed (MEDLINE) for references to ALK
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ALK antibody - more information
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Customer reviews (feedback) regarding ALK antibody |
Customer FAQs regarding ALK antibody |
Protocols for ALK antibody |
Price and availability of products related to ALK antibody |
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ALK antibody - related products:
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ALK antibody - other tools:
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Contact Abcam with a Technical Enquiry about ALK antibody |
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