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Anti-ALK antibody (ab4061)

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Overview

Product name

Anti-ALK antibody
See all ALK products (14) ...

Description

Rabbit polyclonal to ALK

Specificity

Recognizes a human p80 protein, identified as a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in a third of large cell lymphomas. This rebbit polyclonal antibody can be used to detect p80 in these lymphomas and may also be used to detect a recently described subtype of large B cell lymphoma, which expresses the full-length ALK protein.

Tested applications

IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the c-terminus of the NPM-Alk transcript. The exact sequence is considered commercially sensitive.

SEGTS PGSKMALQSS FTCWNGTVLQ LGQACDFHQD C AQGEDESQM CRKLPVGFYC NFEDGFCGWT QGTLSPHTP Q WQVRTLKDAR FQDHQDHALL LSTTDVPA

Positive control

Anaplastic lymphoma.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Polyclonal

Isotype

IgG

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ALK antibody(ab4061)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ALK antibody(ab4061) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab4061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Orphan receptor with a tyrosine-protein kinase activity. Appears to play an important role in the normal development and function of the nervous system. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif.

Tissue specificity

Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.

Involvement in disease

Note=A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas.
Note=A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A.
Note=A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.
Defects in ALK are the cause of susceptibility to neuroblastoma type 3 (NBLST3) [MIM:613014]. Neuroblastoma is a common neoplasm of early childhood arising from embryonic cells that form the primitive neural crest and give rise to the adrenal medulla and the sympathetic nervous system.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 1 LDL-receptor class A domain.
Contains 2 MAM domains.
Contains 1 protein kinase domain.

Post-translational
modifications

N-glycosylated.

Cellular localization

Membrane.

Target information above from: UniProt accessionQ9UM73 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Alk antibody
  • ALK tyrosine kinase receptor antibody
  • ALK Tyrosine Kinase Receptor Precursor antibody
  • ALK/EML4 fusion gene, included antibody
  • ALK/NPM1 fusion gene, included antibody
  • ALK_HUMAN antibody
  • anaplastic lymphoma kinase (Ki-1) antibody
  • Anaplastic lymphoma kinase antibody
  • Anaplastic lymphoma kinase Ki1 antibody
  • Anaplastic Lymphoma Kinase p80 antibody
  • CD246 antibody
  • CD246 Antigen antibody
  • EC 2.7.10.1 antibody
  • Ki 1 antibody
  • Ki1 antibody
  • NBLST3 antibody
  • Tcrz antibody
  • TFG/ALK antibody
see all

Anti-ALK antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ALK antibody(ab4061)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ALK antibody(ab4061)

Ab4061 staining Human normal colon. Staining is localized to nucleus and membrane.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-ALK antibody (ab4061)

ab4061 has not yet been referenced specifically in any publications.

Publishing research using ab4061? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"