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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Overview
Product name
Anti-ALK antibody
See all ALK products (14) ...
Description
Rabbit polyclonal to ALK
Specificity
Recognizes a human p80 protein, identified as a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in a third of large cell lymphomas. This rebbit polyclonal antibody can be used to detect p80 in these lymphomas and may also be used to detect a recently described subtype of large B cell lymphoma, which expresses the full-length ALK protein.
Tested applications
IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the c-terminus of the NPM-Alk transcript. The exact sequence is considered commercially sensitive.
SEGTS PGSKMALQSS FTCWNGTVLQ LGQACDFHQD C AQGEDESQM CRKLPVGFYC NFEDGFCGWT QGTLSPHTP Q WQVRTLKDAR FQDHQDHALL LSTTDVPA
Positive control
Anaplastic lymphoma.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ALK antibody(ab4061)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab4061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 2 µg/ml
IHC-Fr: Use at an assay dependent dilution.
Target
Function
Orphan receptor with a tyrosine-protein kinase activity. Appears to play an important role in the normal development and function of the nervous system. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif.
Tissue specificity
Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.
Involvement in disease
Note=A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas.
Note=A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A.
Note=A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.
Defects in ALK are the cause of susceptibility to neuroblastoma type 3 (NBLST3) [MIM:613014]. Neuroblastoma is a common neoplasm of early childhood arising from embryonic cells that form the primitive neural crest and give rise to the adrenal medulla and the sympathetic nervous system.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 1 LDL-receptor class A domain.
Contains 2 MAM domains.
Contains 1 protein kinase domain.
Post-translational
modifications
N-glycosylated.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ9UM73
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alk antibody
- ALK tyrosine kinase receptor antibody
- ALK Tyrosine Kinase Receptor Precursor antibody
see all
Database links
- Entrez Gene: 238 Human
- Omim: 105590 Human
- SwissProt: Q9UM73 Human
- Unigene: 654469 Human
Anti-ALK antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ALK antibody(ab4061)
Ab4061 staining Human normal colon. Staining is localized to nucleus and membrane.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-ALK antibody (ab4061)
ab4061 has not yet been referenced specifically in any publications.
Publishing research using ab4061? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"