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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ALK antibody(ab4061)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ALK antibody(ab4061)
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Product Name 

ALK antibody

 

See all ALK antibodies (12)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ALK

Immunogen 

Recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the c-terminus of the NPM-Alk transcript. The exact sequence is considered commercially sensitive.
BLAST 'SEGTS PGSK MALQSS FTC WNGTVLQ LG QACDFHQD C AQGEDESQM CRKLPVGFYC NFEDGFCGWT QGTLSPHTP Q WQVRTLKD AR FQDHQDH ALL LSTTDV ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Specificity 

Recognizes a human p80 protein, identified as a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in a third of large cell lymphomas. This rebbit polyclonal antibody can be used to detect p80 in these lymphomas and may also be used to detect a recently described subtype of large B cell lymphoma, which expresses the full-length ALK protein.

Tested applications 
(see key)

IHC-Fr, IHC-P


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
IHC-P: Use at a concentration of 2 µg/ml.
IHC-Fr: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Anaplastic lymphoma.

Cellular localization 

Type I membrane protein

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases

Relevance 

Anaplastic lymphoma kinase (ALK) was originally discovered as a NPM (Nucleophosmin)-ALK fusion protein. ALK and LTK (leukocyte tyrosine kinase) are receptor tyrosine kinases similar to the insulin receptor subfamily of kinases. LTK is found in murine B Lymphocyte precursors and in forebrain neurons. ALK is usually found in the nervous system. A truncated form of ALK occurs in many non Hodgkin's lymphomas.
The ALK gene is on chromosome 2. Upon translocation between chromosome 2 and chromosome 5 [t(2;5}], the ALK gene fuses with the NPM gene. NPM, also known as B23, is an ubiquitous nucleolar phosphoprotein involved in the transport of pre ribosomal components between the nucleolus and the cytoplasm. The chimeric product (NPM ALK) resulting from t(2;5) translocation is a protein of 80kDa with the N terminal portion of NPM linked to the complete intracellular portion of ALK. Karpas 299 and SUDHL1 lymphoid cell lines strongly express NPM ALK.

 

Alternative names ALK antibody (ab4061)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

238    (Human)

Omim

    

105590    (Human)

SwissProt

    

Q9UM73    (Human)

Unigene

    

654469    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Store at +4°C.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ALK antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ALK antibody images, references, products related to ab4061 and other tools.

 

ALK antibody images:

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ALK antibody(ab4061)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ALK antibody(ab4061)

Ab4061 staining Human normal colon. Staining is localized to nucleus and membrane.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

 
 

Search PubMed (MEDLINE) for references to ALK

 

ALK antibody - more information

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Price and availability of products related to ALK antibody

 

ALK antibody - related products:

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

 

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Other ALK Antibodies

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