Anti-ALK (phospho Y1096) antibody (ab111865)

Overview

• Product nameAnti-ALK (phospho Y1096) antibodySee all ALK primary antibodies ...
• Description
  Rabbit polyclonal to ALK (phospho Y1096)
• Specificityab111865 detects endogenous levels of ALK only when phosphorylated at tyrosine 1096.
• Tested applicationsIHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse
• Immunogen

  Synthetic phosphopeptide derived from Human ALK around the phosphorylation site of tyrosine 1096 (P-N-Y^P-C-F).

• Positive controlHuman breast carcinoma tissue.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab111865 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Growth factor receptors

  • Tags & Cell Markers
  • Cell Type Markers
  • Tumor Associated

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Soma marker

  • Neuroscience
  • Neurotransmission
  • Intracellular Signaling
  • Kinases

  • Neuroscience
  • Neurotransmission
  • Receptors / Channels
  • Tyrosine Kinase Receptors

Applications

Target

• FunctionOrphan receptor with a tyrosine-protein kinase activity. Appears to play an important role in the normal development and function of the nervous system. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif.
• Tissue specificityExpressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.
• Involvement in diseaseNote=A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas.
  Note=A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A.
  Note=A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.
  Defects in ALK are the cause of susceptibility to neuroblastoma type 3 (NBLST3) [MIM:613014]. Neuroblastoma is a common neoplasm of early childhood arising from embryonic cells that form the primitive neural crest and give rise to the adrenal medulla and the sympathetic nervous system.
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
  Contains 1 LDL-receptor class A domain.
  Contains 2 MAM domains.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsN-glycosylated.
• Cellular localizationMembrane.

Target information above from: UniProt accession Q9UM73 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 238 Human
  • Entrez Gene: 11682 Mouse
  • Omim: 105590 Human
  • SwissProt: Q9UM73 Human
  • SwissProt: P97793 Mouse
  • Unigene: 654469 Human
  • Unigene: 311854 Mouse

• Alternative names
  Alk antibodyALK tyrosine kinase receptor antibodyALK/EML4 fusion gene, included antibody

  ALK/NPM1 fusion gene, included antibodyALK_HUMAN antibodyanaplastic lymphoma kinase (Ki-1) antibodyAnaplastic lymphoma kinase antibodyAnaplastic lymphoma kinase Ki1 antibodyanaplastic lymphoma receptor tyrosine kinase antibodyCD 246 antibodyCD246 antibodyCD246 antigen antibodyEC 2.7.10.1 antibodyKi 1 antibodyKi1 antibodymutant anaplastic lymphoma kinase antibodyNBLST 3 antibodyNBLST3 antibodyTcrz antibodyTFG/ALK antibody

  see all

Anti-ALK (phospho Y1096) antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ALK (phospho Y1096) antibody (ab111865)
  ab111865 at 1/50 dilution staining ALK in paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.