Anti-ALK (phospho Y1507) antibody (ab73996)
Key features and details
- Rabbit polyclonal to ALK (phospho Y1507)
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-ALK (phospho Y1507) antibody
See all ALK primary antibodies -
Description
Rabbit polyclonal to ALK (phospho Y1507) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human ALK (phospho Y1507).
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Positive control
- human brain tissue, extracts from COS cells, treated with anisomycin (25ug/ml, 30mins)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 176 kDa (predicted molecular weight: 176 kDa).
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 176 kDa (predicted molecular weight: 176 kDa). |
IHC-P
1/50 - 1/100. |
Target
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Function
Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK. -
Tissue specificity
Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells. -
Involvement in disease
A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas.
A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A.
A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.
Neuroblastoma 3
The ALK signaling pathway plays an important role in glioblastoma, the most common malignant brain tumor of adults and one of the most lethal cancers. It regulates both glioblastoma migration and growth.
A chromosomal aberration involving ALK is found in one subject with colorectal cancer. Translocation t(2;2)(p23.1;p23.3). A 5 million base pair tandem duplication generates an in-frame WDCP-ALK gene fusion. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 1 LDL-receptor class A domain.
Contains 2 MAM domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. In cells not stimulated by a ligand, receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) dephosphorylates ALK at the sites in ALK that are undergoing autophosphorylation through autoactivation. Phosphorylation at Tyr-1507 is critical for SHC1 association.
N-glycosylated. -
Cellular localization
Cell membrane. Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway. - Information by UniProt
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Database links
- Entrez Gene: 238 Human
- Entrez Gene: 11682 Mouse
- Omim: 105590 Human
- SwissProt: Q9UM73 Human
- SwissProt: P97793 Mouse
- Unigene: 654469 Human
- Unigene: 311854 Mouse
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Alternative names
- Alk antibody
- ALK tyrosine kinase receptor antibody
- ALK/EML4 fusion gene, included antibody
see all
Images
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All lanes : Anti-ALK (phospho Y1507) antibody (ab73996) at 1/500 dilution
Lane 1 : extracts from COS cells, treated
with anisomycin (25ug/ml, 30mins)
Lane 2 : extracts from COS cells, treated
with anisomycin (25ug/ml, 30mins) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 176 kDa
Observed band size: 176 kDa -
Immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab73996. Left: untreated sample. Right: sample treated with immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab73996 has been referenced in 6 publications.
- Yu X et al. MDK induces temozolomide resistance in glioblastoma by promoting cancer stem-like properties. Am J Cancer Res 12:4825-4839 (2022). PubMed: 36381313
- Huang H et al. Extracellular domain shedding of the ALK receptor mediates neuroblastoma cell migration. Cell Rep 36:109363 (2021). PubMed: 34260934
- Cohen MA et al. Formation of Human Neuroblastoma in Mouse-Human Neural Crest Chimeras. Cell Stem Cell 26:579-592.e6 (2020). PubMed: 32142683
- Debruyne DN et al. BORIS promotes chromatin regulatory interactions in treatment-resistant cancer cells. Nature 572:676-680 (2019). PubMed: 31391581
- Yoo SK et al. Comprehensive Analysis of the Transcriptional and Mutational Landscape of Follicular and Papillary Thyroid Cancers. PLoS Genet 12:e1006239 (2016). PubMed: 27494611
- Koh J et al. EML4-ALK enhances programmed cell death-ligand 1 expression in pulmonary adenocarcinoma via hypoxia-inducible factor (HIF)-1a and STAT3. Oncoimmunology 5:e1108514 (2016). PubMed: 27141364