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All lanes : Anti-ALK (phospho Y1604) antibody [EP661Y] (ab51030) at 1/5000 dilution
Lane 1 : SH-SY-5Y cell lysate, untreated
Lane 2 : SH-SY-5Y cell lysate, treated with PTN
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 176 kDa
Observed band size : 220 kDa (why is the actual band size different from the predicted?)
Ab51030 (1:100) staining human ALK in human Non-Hodgkins lymphoma tissue by immunohistochemistry using paraffin embedded tissue.
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