ALK (phospho Y1604) antibody [EP661Y] (ab51030)

Product Name 

ALK (phospho Y1604) antibody [EP661Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP661Y] to ALK (phospho Y1604)

Immunogen 

Synthetic phosphor-peptide corresponding to residues surrounding Tyr1604 of human ALK.

Reacts with 

Hu

Does not react with 
(species key)

Ms, Rat

Specificity 

This antibody only detects ALK phosphorylated on Tyrosine 1604.

Tested applications 
(see key)

IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/100 - 1/250. We strongly recommend that customers perform an antigen retrieval step.
IP: 1/20.
WB: 1/5000. Detects a band of approximately 220 kDa (predicted molecular weight: 176 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

SH-SY-5Y cell lysates or human Non-Hodgkins lymphoma tissue.

Cellular localization 

Type I membrane protein

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases

Relevance 

Anaplastic lymphoma kinase (ALK) was originally discovered as a NPM (Nucleophosmin)-ALK fusion protein. ALK and LTK (leukocyte tyrosine kinase) are receptor tyrosine kinases similar to the insulin receptor subfamily of kinases. LTK is found in murine B Lymphocyte precursors and in forebrain neurons. ALK is usually found in the nervous system. A truncated form of ALK occurs in many non Hodgkin's lymphomas.
The ALK gene is on chromosome 2. Upon translocation between chromosome 2 and chromosome 5 [t(2;5}], the ALK gene fuses with the NPM gene. NPM, also known as B23, is an ubiquitous nucleolar phosphoprotein involved in the transport of pre ribosomal components between the nucleolus and the cytoplasm. The chimeric product (NPM ALK) resulting from t(2;5) translocation is a protein of 80kDa with the N terminal portion of NPM linked to the complete intracellular portion of ALK. Karpas 299 and SUDHL1 lymphoid cell lines strongly express NPM ALK.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP661Y

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

Notes 

Produced under U.S. Patent No. 5,675,063.

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