Anti-ALKBH3 antibody (ab56540)

Overview

  • Product nameAnti-ALKBH3 antibodySee all ALKBH3 primary antibodies ...
  • Description
    Mouse monoclonal to ALKBH3
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-140 of Human ALKBH3

Properties

Applications

Our Abpromise guarantee covers the use of ab56540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDioxygenase that repairs alkylated DNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Has a strong preference for single-stranded DNA. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron.
    • Tissue specificityUbiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon.
    • Sequence similaritiesBelongs to the alkB family.
      Contains 1 Fe2OG dioxygenase domain.
    • Cellular localizationCytoplasm. Nucleus.
    • Target information above from: UniProt accession Q96Q83 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • 1700108H04Rik antibody
      • 1810020C19Rik antibody
      • ABH3 antibody
      • AlkB homolog 3 antibody
      • alkB, alkylation repair homolog 3 (E. coli) antibody
      • ALKB3_HUMAN antibody
      • ALKBH3 antibody
      • Alkylated DNA repair protein alkB homolog 3 antibody
      • alkylation repair homolog 3 antibody
      • Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 antibody
      • DEPC 1 antibody
      • DEPC-1 antibody
      • DEPC1 antibody
      • EC 1.14.11. antibody
      • FLJ43614 antibody
      • mABH3 antibody
      • MGC118790 antibody
      • MGC118792 antibody
      • MGC118793 antibody
      • MGC134125 antibody
      • PCA1 antibody
      • Prostate cancer antigen 1 antibody
      • Prostate cancer antigen-1 antibody
      • RP23-375N21.4 antibody
      see all

    Anti-ALKBH3 antibody images



    • Predicted band size : 33 kDa
      ALKBH3 antibody (ab56540) at 1ug/lane + K-562 cell lysate at 25ug/lane.

    References for Anti-ALKBH3 antibody (ab56540)

    ab56540 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you very much for your reply. My colleague is out of the office today but I'll be happy to help you with this.

    We don't currently have blocking peptides available for ab105340 or ab56540, but I'm in the process of adding the peptide for...

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    Thank you for your reply.

    I hope that the positive control lysate will give clearer results for you. Based on your protocol, the only suggestion I would make would be to boil your samples for 5 minutes to ensure they are fully reduced and den...

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    Thank you for your reply. It would be very helpful if you could please provide the below information about your protocol:

    1) What type of samples are you testing? What species are they from, what lysis buffer was used, were they prepared with...

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    Thank you for your reply.

    As a positive control for both ab56540 and ab105340 I would recommend K-562 cell lysate (ab7911). Since both proteins are very widely expressed, I am not able to recommend a sample that would work as a negative contr...

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    Thank you for your reply. The publications that are known to reference an Abcam product can be found on our online datasheets on the specific references tab. Below are PubMed IDs for the antibodies you are interested in.

    MGMT (ab39253) - 2175...

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    Thank you for contacting us.

    The final dilutions should be optimized for your particular samples and protocols, but the following would be my recommended starting dilutions for WB with an overnight incubation:

    MGMT (ab39253) - 1:500...

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    Application Western blot
    Sample Human Cell lysate - whole cell (Hep G2)
    Loading amount 10000 cells
    Specification Hep G2
    Treatment plasmids transfected cells
    Gel Running Conditions Reduced Denaturing (4-12%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Dr. Shijian Zhang

    Verified customer

    Submitted Nov 07 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"