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Anti-ALKBH3 antibody (ab56540)

Abreviews (1)Q&A (6)

Overview

  • Product nameAnti-ALKBH3 antibodySee all ALKBH3 primary antibodies ...
  • Description
    Mouse monoclonal to ALKBH3
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-140 of Human ALKBH3

Properties

Applications

Our Abpromise guarantee covers the use of ab56540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionDioxygenase that repairs alkylated DNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Has a strong preference for single-stranded DNA. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron.
    • Tissue specificityUbiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon.
    • Sequence similaritiesBelongs to the alkB family.
      Contains 1 Fe2OG dioxygenase domain.
    • Cellular localizationCytoplasm. Nucleus.

      • Target information above from: UniProt accession Q96Q83 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          1700108H04Rik antibody1810020C19Rik antibodyABH3 antibody
          AlkB homolog 3 antibodyalkB, alkylation repair homolog 3 (E. coli) antibodyALKB3_HUMAN antibodyALKBH3 antibodyAlkylated DNA repair protein alkB homolog 3 antibodyalkylation repair homolog 3 antibodyAlpha-ketoglutarate-dependent dioxygenase alkB homolog 3 antibodyDEPC 1 antibodyDEPC-1 antibodyDEPC1 antibodyEC 1.14.11. antibodyFLJ43614 antibodymABH3 antibodyMGC118790 antibodyMGC118792 antibodyMGC118793 antibodyMGC134125 antibodyPCA1 antibodyProstate cancer antigen 1 antibodyProstate cancer antigen-1 antibodyRP23-375N21.4 antibody
        see all

      Anti-ALKBH3 antibody images

      • Western blot - ALKBH3 antibody (ab56540)
        Western blot - ALKBH3 antibody (ab56540)


        Predicted band size : 33 kDa
        ALKBH3 antibody (ab56540) at 1ug/lane + K-562 cell lysate at 25ug/lane.

      References for Anti-ALKBH3 antibody (ab56540)

      ab56540 has not yet been referenced specifically in any publications.

      Publishing research using ab56540 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab56540 Submit a Question for ab56540

      Displaying 1 - 7 of 7 results for Abreviews and Q&A

      10
      Votes: Highest First

      And how about the locking peptide?

      Do you have it? And how would that work? Can you explain?

      Thank you very much for your reply. My colleague is out of the office today but I'll be happy to help you with this.

      We don't currently have blocking peptides available for ab105340 or ab56540, but I'm in the process of adding the peptide for ab...

      Read More

      Thanks so much for sending your questions.

      Here are my answers:

      1) Human cell lines (HT1080 - colangiocarcinoma, U87- glioma and LN18 - glioma)

      They are all lysed in RIPA buffer:

      50 mM Tris-HCl (pH8.8)

      Read More

      Thank you for your reply.

      I hope that the positive control lysate will give clearer results for you. Based on your protocol, the only suggestion I would make would be to boil your samples for 5 minutes to ensure they are fully reduced and denatu...

      Read More

      what exactly do you need to know
      send questions and I will answer them all promptly.

      Thank you for your reply. It would be very helpful if you could please provide the below information about your protocol:

      1) What type of samples are you testing? What species are they from, what lysis buffer was used, were they prepared with pr...

      Read More

      Thanks for your previous suggestions.

      I have another question.

      I am working with these two antibodies:

      ABH2 (ab105340)

      ABH3 (ab56540)

      Please have a look at attached western blots.

      I am look...

      Read More

      Thank you for your reply.

      As a positive control for both ab56540 and ab105340 I would recommend K-562 cell lysate (ab7911). Since both proteins are very widely expressed, I am not able to recommend a sample that would work as a negative control ...

      Read More

      Do you, perhaps, have any reference to a published work in which these antibodies were applied?

      Thank you for your reply. The publications that are known to reference an Abcam product can be found on our online datasheets on the specific references tab. Below are PubMed IDs for the antibodies you are interested in.

      MGMT (ab39253) - 2175228...

      Read More

      I am working with these antibodies.
      MGMT
      ABH2
      APNG
      ABH3
      Can you please advise on final dilution to use for ON +4C incubation in 5% milk/PBST?

      Thank you for contacting us.

      The final dilutions should be optimized for your particular samples and protocols, but the following would be my recommended starting dilutions for WB with an overnight incubation:

      MGMT (ab39253) - 1:500 Read More

      Western blot abreview for Anti-ALKBH3 antibody

       Excellent
      Abreviews
      Application Western blot
      Sample Human Cell lysate - whole cell (Hep G2)
      Loading amount 10000 cells
      Specification Hep G2
      Treatment plasmids transfected cells
      Gel Running Conditions Reduced Denaturing (4-12%)
      Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
      Read More
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      Submitted Nov 07 2011

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"