Overview

  • Product nameAnti-ALKBH3 antibody
    See all ALKBH3 primary antibodies
  • Description
    Rabbit polyclonal to ALKBH3
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 215-264 (EMRKKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADWQHRVPKEYH S) of Human ALKBH3, NP_631917

  • Positive control
    • Fetal muscle lysate (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab85636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionDioxygenase that repairs alkylated DNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Has a strong preference for single-stranded DNA. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron.
  • Tissue specificityUbiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon.
  • Sequence similaritiesBelongs to the alkB family.
    Contains 1 Fe2OG dioxygenase domain.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1700108H04Rik antibody
    • 1810020C19Rik antibody
    • ABH3 antibody
    • AlkB homolog 3 antibody
    • alkB, alkylation repair homolog 3 (E. coli) antibody
    • ALKB3_HUMAN antibody
    • ALKBH3 antibody
    • Alkylated DNA repair protein alkB homolog 3 antibody
    • alkylation repair homolog 3 antibody
    • Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 antibody
    • DEPC 1 antibody
    • DEPC-1 antibody
    • DEPC1 antibody
    • EC 1.14.11. antibody
    • FLJ43614 antibody
    • mABH3 antibody
    • MGC118790 antibody
    • MGC118792 antibody
    • MGC118793 antibody
    • MGC134125 antibody
    • PCA1 antibody
    • Prostate cancer antigen 1 antibody
    • Prostate cancer antigen-1 antibody
    • RP23-375N21.4 antibody
    see all

Anti-ALKBH3 antibody images

  • Anti-ALKBH3 antibody (ab85636) at 1 µg/ml + Fetal muscle lysate (Human) at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ALKBH3 antibody (ab85636)

ab85636 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"