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Overview

  • Product nameALS2CR1 protein (Tagged-His Tag)See all ALS2CR1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 101-350 (250 amino acids) of Human ALS2CR1 (Isoform 2) with N terminal His tag; Predicted MWt 28 kDa (UniProt Q9GZT8-2).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 93 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesTAYDAAPQGVNNWLAKGLGACTSRPIHPSKAPNYPTEGNH RVEFNVNYT QDLDKVMSAVKGIDGVSVTSFSARTGNEE QTRINLNCTQKALMQVVDFL SRNKQLYQKTEILSLEKP LLLHTGMGRLCTLDESVSLATMIDRIKRHLK LSHIRLA LGVGRTLESQVKVVALCAGSGSSVLQGVEADLYLTGEMSH HD TLDAASQGINVILCEHSNTERGFLSDLRDMLDSHLE NKINIILSETDRD PLQVV
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92196 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        ALS2CR1Amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 1Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 1 protein
        CALS-7MDS015Ngg1 interacting factor 3-like 1NIF3 (Ngg1 interacting factor 3, S.pombe homolog)-like 1NIF3 NGG1 interacting factor 3-like 1NIF3 NGG1 interacting factor 3-like 1 (S. pombe)NIF3-like protein 1NIF3L_HUMANNIF3L1OTTHUMP00000206190OTTHUMP00000206191OTTHUMP00000206192
      see all
  • Sequence similaritiesBelongs to the UPF0135 (NIF3) family.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession Q9GZT8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    ALS2CR1 protein (Tagged-His Tag) images

    • SDS-PAGE - ALS2CR1 protein (Tagged-His Tag) (ab92196)
      SDS-PAGE - ALS2CR1 protein (Tagged-His Tag) (ab92196)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for ALS2CR1 protein (Tagged-His Tag) (ab92196)

    ab92196 has not yet been referenced specifically in any publications.

    Publishing research using ab92196 ? Please let us know so that we can cite the reference in this datasheet

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