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ab6330 staining AMACR by immunohistochemistry on paraffin-embedded human normal prostate tissue (left, light staining) and on prostate adenocarcinoma using DAB staining (right)
ab63340 at 1/1000 dilution staining AMACR in LnCap cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated goat anti-mouse {IgG (H+C)} was used as secondary. Actin filaments were stained red with DY-554 phalloidin. Nuclei were stained blue with DRAQ5 fluorescent DNA dye.
All lanes : Anti-AMACR antibody [2A10F3] (ab63340) at 1/2000 dilution
Lane 1 : Cell lysates prepared from Jurkat cells
Lane 2 : Cell lysates prepared from LnCaP cells
Lysates/proteins at 100 µg per lane.
Secondary
HRP-conjugated Goat polyclonal to mouse IgG1
Predicted band size : 42 kDa
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