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Anti-AMACR antibody [2A10F3] (ab63340)

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2 questions for ab63340

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Question 1

Thursday 26-January-2012

Dear All,



Please let us know about the following that many slides should have been available for the concentrated antibodies after dilution and how many slides can be prepeared with the prediluted slides. Also let us know the about the deteation kit and DAB ab64264 and



ab3223

ab63340

ab74179

ab8105

ab27595 (pre-diluted)

ab27596 (pre-diluted)

ab15467 (pre-diluted)


ab64264 -sufficient for how many slides?

Awaiting your early response.

Sincere regards,

ANSWER:

 

Thank you for your inquiry.

Since the working dilutions of antibodies are not a fixed value but have to optimised by the endusers themselves according to experimental condition, I cannot give you absolute numbers. Even when the pre-diluted antibodies are used neat there might be reasons to use 50ul per slide or maybe even 100ul per slide.

These conditions include: size of the sample, tissue type, lab experience, diluent buffer, species, antigen retrieval, incubation time, etc.

For the products you mentioned I can give a estimate for the pre-diluted antibodies of ca 140- 280slides, for the other antibodies 500 - 100 slides and for the detection kit 150 - 300 slides.

I hope this information is helpful.

Question 2

Friday 30-September-2011

Dear all

We have a customer that wants the antibody RACEMACE for IHC, witch is the best clone from ABCAM to do it ??

Regards

ANSWER:

 

Thank you for contacting us.

Could you please suggest following products to your customer. These antibodies are against alpha Methylacyl-CoA Racemace target. It may be that the customer is looking for the same product. The catalogue numbers are

ab63340; ab15893; ab94944; ab15894; ab93406; ab69455; ab7693; ab12498; ab93045; ab82747 and ab14202.

Please also check the alternative names of these products given in the datasheets.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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