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Western blot - AMACR antibody [2A10F3] (ab63340)
![Western blot - AMACR antibody [2A10F3] (ab63340)](/ps/datasheet/images/ab63340_wb.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMACR antibody [2A10F3] (ab63340)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMACR antibody [2A10F3] (ab63340)](/ps/datasheet/images/ab63340_ihc2.jpg)
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Product Name
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AMACR antibody [2A10F3]
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See all AMACR antibodies (7)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [2A10F3] to AMACR
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Immunogen
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Purified truncated recombinant AMACR-His fusion protein expressed in E. Coli strain BL21 (DE3)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
IHC-P: 1/500 - 1/2000.
WB: 1/500 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat Cell lysate. Prostate adenocarcinoma.
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Cellular localization
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Mitochondrial and Peroxisomal.
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Relevance
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AMARC has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMARC protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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2A10F3
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Isotype
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IgG2b
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Purity
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Ascites
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Storage buffer
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Preservative: None
Constituents: Ascites
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AMACR antibody [2A10F3] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AMACR antibody [2A10F3] images, references, products related to ab63340 and other tools.
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AMACR antibody [2A10F3] images:
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 Western blot - AMACR antibody [2A10F3] (ab63340)
All lanes : AMACR antibody [2A10F3] (ab63340) at 1/500 dilution
Lane 1 : truncated AMACR-His recombinant protein
Lane 2 : Jurkat cell lysate
Predicted band size : 42 kDa
Observed band size : 42 kDa
Additional bands at : 26 kDa (possible truncated form).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMACR antibody [2A10F3] (ab63340)
ab6330 staining AMACR by immunohistochemistry on paraffin-embedded human normal prostate tissue (left, light staining) and on prostate adenocarcinoma using DAB staining (right)
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Search PubMed (MEDLINE) for references to AMACR
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AMACR antibody [2A10F3] - more information
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Customer reviews (feedback) regarding AMACR antibody [2A10F3] |
Customer FAQs regarding AMACR antibody [2A10F3] |
Protocols for AMACR antibody [2A10F3] |
Price and availability of products related to AMACR antibody [2A10F3] |
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AMACR antibody [2A10F3] - related products:
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AMACR antibody [2A10F3] - other tools:
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Contact Abcam with a Technical Enquiry about AMACR antibody [2A10F3] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - AMACR antibody [2A10F3] (ab63340)](/index.html?datasheet=63340#ab63340_wb.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMACR antibody [2A10F3] (ab63340)](/index.html?datasheet=63340#ab63340_ihc2.jpg)
