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Read our guarantee »Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Anti-AMACR antibody [2A10F3]
See all AMACR products (9) ...
Mouse monoclonal [2A10F3] to AMACR
ICC/IF, WB, ELISA, IHC-Pmore details
Reacts with
Human
Purified truncated recombinant AMACR-His fusion protein expressed in E. Coli strain BL21 (DE3)
Jurkat Cell lysate. Prostate adenocarcinoma.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: Ascites
Concentration information loading...
Ascites
Monoclonal
2A10F3
IgG2b
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Our Abpromise guarantee covers the use of ab63340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/200 - 1/1000.
WB: 1/500 - 1/2000.Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ELISA: 1/10000
IHC-P: 1/500 - 1/2000.
AMARC has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMARC protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia.
Mitochondrial, Peroxisome
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMACR antibody [2A10F3] (ab63340)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMACR antibody [2A10F3] (ab63340)](/ps/datasheet/Images/63/ab63340/ab63340_ihc2.jpg)
ab6330 staining AMACR by immunohistochemistry on paraffin-embedded human normal prostate tissue (left, light staining) and on prostate adenocarcinoma using DAB staining (right)
Immunocytochemistry/ Immunofluorescence - AMACR antibody [2A10F3] (ab63340)
![Immunocytochemistry/ Immunofluorescence - AMACR antibody [2A10F3] (ab63340)](/ps/datasheet/images/63/ab63340/AMACR-Primary-antibodies-ab63340-1.jpg)
ab63340 at 1/1000 dilution staining AMACR in LnCap cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated goat anti-mouse {IgG (H+C)} was used as secondary. Actin filaments were stained red with DY-554 phalloidin. Nuclei were stained blue with DRAQ5 fluorescent DNA dye.
Western blot - AMACR antibody [2A10F3] (ab63340)
![Western blot - AMACR antibody [2A10F3] (ab63340)](/ps/datasheet/images/63/ab63340/AMACR-Primary-antibodies-ab63340-2.jpg)
All lanes : Anti-AMACR antibody [2A10F3] (ab63340) at 1/2000 dilution
Lane 1 : Cell lysates prepared from Jurkat cells
Lane 2 : Cell lysates prepared from LnCaP cells
Lysates/proteins at 100 µg per lane.
Secondary
HRP-conjugated Goat polyclonal to mouse IgG1
Predicted band size : 42 kDa
ab63340 has not yet been referenced specifically in any publications.
Publishing research using ab63340? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMACR antibody [2A10F3] (ab63340)](/ps/datasheet/Images/63/ab63340/ab63340_ihc2.jpg)
ab6330 staining AMACR by immunohistochemistry on paraffin-embedded human normal prostate tissue (left, light staining) and on prostate adenocarcinoma using DAB staining (right)
![Immunocytochemistry/ Immunofluorescence - AMACR antibody [2A10F3] (ab63340)](/ps/datasheet/images/63/ab63340/AMACR-Primary-antibodies-ab63340-1.jpg)
ab63340 at 1/1000 dilution staining AMACR in LnCap cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated goat anti-mouse {IgG (H+C)} was used as secondary. Actin filaments were stained red with DY-554 phalloidin. Nuclei were stained blue with DRAQ5 fluorescent DNA dye.
![Western blot - AMACR antibody [2A10F3] (ab63340)](/ps/datasheet/images/63/ab63340/AMACR-Primary-antibodies-ab63340-2.jpg)
All lanes : Anti-AMACR antibody [2A10F3] (ab63340) at 1/2000 dilution
Lane 1 : Cell lysates prepared from Jurkat cells
Lane 2 : Cell lysates prepared from LnCaP cells
Lysates/proteins at 100 µg per lane.
Secondary
HRP-conjugated Goat polyclonal to mouse IgG1
Predicted band size : 42 kDa
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