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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal (ab14202)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal (ab14202)](/ps/datasheet/images/ab14202_1.jpg)
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Product Name
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AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal
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See all AMACR + p63 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [4A4 (p63)] to AMACR + p63 - Cocktail of mouse monoclonal and rabbit polyclonal
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Immunogen
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Monoclonal : Recombinant fragment corresponding to amino acids 1-205 of Human p63;
Polyclonal: synthetic human AMACR peptide.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/25 - 1/50. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Prostate carcinoma
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Cellular localization
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Nuclear and Mitochondrial
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Research areas
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Cancer >> Tumor biomarkers >> Other
Signal Transduction >> Metabolism >> Lipid metabolism
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Relevance
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AMACR has been recently described as a prostate cancer specific gene that encodes a protein involved in the beta oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN.
The p63 gene is highly expressed in the basal or progenitor layers of many epithelial tissues. P63 shows remarkable structural similarity to p53 gene. Unlike p53, the p63 gene encodes multiple isotypes with remarkable divergent abilities to transactivate p53 reporter genes and induce apoptosis.
The combination of AMACR and p63 (PIN) may be extremely useful for diagnosing prostatic intraepithelial neoplasia (PIN). AMACR stains cytoplasm in prostatic adenocarcinomas and atypical hyperplasia and p63 stains nuclei in normal and benign prostate glands.
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AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal (ab14202) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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4A4 (p63)
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Isotype
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IgG2a
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: Sodium Azide
Constituents: Tissue culture supernatant Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Mouse monoclonal (anti-p63) purity: tissue culture supernatant.
Rabbit polyclonal (anti-AMACR) purity: purified immunoglobulin fraction of rabbit antiserum.
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C.
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Notes
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This antibody is supplied as a cocktail of mouse monoclonal (anti-p63) and rabbit polyclonal (anti-AMACR).
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal images, references, products related to ab14202 and other tools.
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AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal (ab14202)
ab14202 staining human prostate carcinoma by IHC-P.
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References for AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal (ab14202)
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This product has been used in: (two most recent references)
Gaf R et al. Sporadic colorectal adenocarcinomas with high-frequency microsatellite instability. Cancer 89:2025-37 (2000). PubMed: 11066042
This clone has been used in:
If you publish research using ab14202 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to AMACR + p63
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AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal - more information
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Customer reviews (feedback) regarding AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal |
Customer FAQs regarding AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal |
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Price and availability of products related to AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal |
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AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal - related products:
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AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal - other tools:
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Contact Abcam with a Technical Enquiry about AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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