![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal (ab14202) image](#ab14202_1.jpg)
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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Anti-AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal (ab14202)
Overview
Product name
Anti-AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal
See all AMACR + p63 products (2) ...
Description
AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Monoclonal : Recombinant fragment corresponding to amino acids 1-205 of Human p63; Polyclonal: synthetic human AMACR peptide.
Positive control
Prostate carcinoma
General notes
This antibody is supplied as a cocktail of mouse monoclonal (anti-p63) and rabbit polyclonal (anti-AMACR).
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, Tissue culture supernatant
Purity
Tissue culture supernatant
Purification notes
Mouse monoclonal (anti-p63) purity: tissue culture supernatant. Rabbit polyclonal (anti-AMACR) purity: purified immunoglobulin fraction of rabbit antiserum.
Clonality
Monoclonal
Clone number
4A4 (p63)
Isotype
IgG2a
Research areas
Cancer >> Tumor biomarkers >> Other
Signal Transduction >> Metabolism >> Lipid metabolism
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal (ab14202)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal (ab14202) image](/ps/datasheet/Images/14/ab14202/ab14202_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab14202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
Application notes
IHC-P: 1/25 - 1/50. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
AMACR has been recently described as a prostate cancer specific gene that encodes a protein involved in the beta oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. The p63 gene is highly expressed in the basal or progenitor layers of many epithelial tissues. P63 shows remarkable structural similarity to p53 gene. Unlike p53, the p63 gene encodes multiple isotypes with remarkable divergent abilities to transactivate p53 reporter genes and induce apoptosis. The combination of AMACR and p63 (PIN) may be extremely useful for diagnosing prostatic intraepithelial neoplasia (PIN). AMACR stains cytoplasm in prostatic adenocarcinomas and atypical hyperplasia and p63 stains nuclei in normal and benign prostate glands.
Cellular localization
Nuclear and Mitochondrial
Alternative names
- 2 methylacyl CoA racemase antibody
- Alpha Methylacyl CoA Racemase antibody
- Alpha Methylacyl CoA Racemase Isoform 1 antibody
see all
Database links
- Entrez Gene: 23600 Human
- Entrez Gene: 8626 Human
- Omim: 603273 Human
- Omim: 604489 Human
- SwissProt: Q96GH1 Human
- SwissProt: Q9H3D4 Human
- Unigene: 137569 Human
- Unigene: 508343 Human
Anti-AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal (ab14202)
ab14202 staining human prostate carcinoma by IHC-P.
References for Anti-AMACR + p63 antibody [4A4 (p63)] - Cocktail of mouse monoclonal and rabbit polyclonal (ab14202)
This product has been referenced in:
- Lanza Get al. Immunohistochemical pattern of MLH1/MSH2 expression is related to clinical and pathological features in colorectal adenocarcinomas with microsatellite instability. Mod Pathol 15:741-9 (2002).Read more (PubMed: 12118112) »
- Gafà Ret al. Sporadic colorectal adenocarcinomas with high-frequency microsatellite instability. Cancer 89:2025-37 (2000).Read more (PubMed: 11066042) »
See all 2 publications for this product
Publishing research using ab14202? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"